精氨酸对子宫内发育迟缓仔猪抗氧化功能和精氨酸代谢的影响  

Effects of supplement of arginine on anti-oxidation and arginine metabolism in intrauterine growth retardation piglets

作　　者：张莉莉[1] 王远孝[1] 孔一力[1] 周根来[1] 王超[1] 钟翔[1] 王恬[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2017年第6期1111-1118,共8页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31572418);国家973计划项目(2012CB124703)

摘　　要：[目的]本文旨在研究精氨酸对子宫内发育迟缓(IUGR)仔猪抗氧化功能和精氨酸代谢的影响。[方法]选择6头胎次和体况相近的妊娠母猪,分娩后,分别从每头母猪所产仔猪中挑选1头正常初生体质量(NBM)新生仔猪和2头IUGR新生仔猪,即6头NBM新生仔猪和12头IUGR新生仔猪。所有IUGR仔猪随机分成2组,分别饲喂基础人工乳(IUGR组,n=6)和添加6 g·L-1精氨酸(Arg)的基础人工乳(IUGR+Arg组,n=6),所有NBM仔猪饲喂基础人工乳(NBM组,n=6),试验期7 d。[结果]与IUGR组相比,日粮补充Arg均显著降低IUGR仔猪背最长肌和空肠黏膜丙二醛(MDA)含量(P<0.05);显著提高空肠黏膜总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GPx)活性(P<0.05);显著提高血清一氧化氮(NO)水平、总NO合酶(TNOS)、诱导型NO合酶(i NOS)、结构型NO合酶(c NOS)活性,空肠黏膜NO水平、TNOS和c NOS活性,以及空肠黏膜与血清中Arg水平和Arg代谢相关氨基酸(Arg、Cit和Pro)水平。[结论]在早期断奶IUGR仔猪日粮中添加精氨酸具有增强IUGR仔猪抗氧化功能和促进精氨酸代谢的作用。[Objectives]The paper aims to evaluate the effect of supplement of arginine（ Arg）on anti-oxidation and arginine metabo- lism in intrauterine growth retardation（IUGR） piglets. [ Methods] Six pregnant sows with the similar parity and body condition were selected, and after parturition, six normal body mass （NBM）piglets and twelve IUGR piglets were selected according to birth weight. All piglets were weaned at 7 d of age, and assigned equally to three groups ： six NBM （ NBM group） and six IUGR piglets （ IUGR group） fed control diet,and six IUGR piglets fed control diet supplemented with 0.6% Arg（ IUGR＋Arg group）for 7 days. [ Results] Compared with IUGR group, the diets supplemented with Arg decreased markedly （P〈O.05）the content of MDA in longissimus dorsi muscle and jejunum mueosa, significantly （ P 〈 O. 05 ） increased T-AOC and the activity of GPx in jejunum mucosa, serum concentration of NO and the activities of TNOS, iNOS, eNOS, the concentration of NO and the activities of TNOS, eNOS in jejunum mucosal, and the serum and mucosal concentrations of Arg, Cit, and Pro in IUGR＋Arg group piglets. [ Conclusions ] Arg plays impor- tant roles in promoting antioxidant function and arginine metabolism in IUGR piglets.

关 键 词：精氨酸 子宫内发育迟缓 抗氧化 精氨酸代谢 仔猪 

分 类 号：S828.9[农业科学—畜牧学]