Lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologies  

作　　者：Guangdun Peng Patrick P.L.Tam Naihe Jing 

机构地区：[1]State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences,University of Chinese Academy of Sciences [2]Embryology Unit, Children’s Medical Research Institute, School of Medical Sciences,Sydney Medical School, University of Sydney [3]School of Life Science and Technology,Shanghai Tech University

出　　处：《National Science Review》2017年第4期533-542,共10页国家科学评论（英文版）

基　　金：supported in part by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01010201);National Key Basic Research and Development Program of China (2014CB964804, 2015CB964500);National Natural Science Foundation of China (31430058, 31571513, 31630043, 91519314, 31661143042);supported by NHMRC Grant 1110751

摘　　要：Establishment of progenitor cell populations and lineage diversity during embryogenesis and the differentiation of pluripotent stem cells is a fascinating and intricate biological process. Conceptually, an understanding of this developmental process provides a framework to integrate stem-cell pluripotency, cell competence and differentiating potential with the activity of extrinsic and intrinsic molecular determinants.The recent advent of enabling technologies of high-resolution transcriptome analysis at the cellular,population and spatial levels proffers the capability of gaining deeper insights into the attributes of the gene regulatory network and molecular signaling in lineage specification and differentiation. In this review, we provide a snapshot of the emerging enabling genomic technologies that contribute to the study of development and stem-cell biology.Establishment of progenitor ceil populations and lineage diversity during embryogenesis and the differentiation ofpluripotent stem cells is a fascinating and intricate biological process. Conceptually, an understanding of this developmental process provides a framework to integrate stem-cell pluripotency, cell competence and differentiating potential with the activity of extrinsic and intrinsic molecular determinants The recent advent of enabling technologies of high-resolution transcriptome analysis at the cellular, population and spatial levels proffers the capability of gaining deeper insights into the attributes of the gene regulatory network and molecular signaling in lineage specification and differentiation. In this review, we provide a snapshot of the emerging enabling genomic technologies that contribute to the study of development and stem-cell biology.

关 键 词：early development cell lineage cell identity scRNA-seq spatial transcriptome 

分 类 号：Q813[生物学—生物工程]