Effects of Mahuang(Herba Ephedra Sinica) and Wuweizi(Fructus Schisandrae Chinensis) medicated serum on chemotactic migration of alveolar macrophages and inters regions macrophages in rats  

作　　者：Yuan Yiping Zhai Huaqiang Zhang Tian Guo Zhaojuan Kong Liting Jia Xiaoyu Wang Ningning Dai Ying Liu Guoxiu Wang Yanping 

机构地区：[1]Department of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese Medicine [2]Department of Pharmacy, Beijing Hospital [3]Institute of Clinical Basic Medicine of Traditional Chinese Medicine, Chinese Academy of Traditional Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》2017年第5期607-615,共9页中医杂志（英文版）

基　　金：Supported by The National Natural Science Foundation of China(No.81373887);The Traditional Chinese Medicine Standardization Project of the State Administration of Traditional Chinese Medicine(No.ZYXX-2014);The Beijing Municipal Administration of Traditional Chinese Medicine"Generation 3+3"Project-Jin Shiyuan the Well-Known Old Man Chinese Medicine Studio Station(Beijing University of Chinese Medicine)Construction Project

摘　　要：OBJECTIVE: To observe the effects of serum containing Mahuang(Herba Ephedra Sinica) or Wuweizi(Fructus Schisandrae Chinensis) on the migration of alveolar macrophages(AM) and interstitial macrophages(IM) from normal rats, and to analyze and compare the mechanisms leading to cell migration differences.METHODS: Rats were randomly divided into three groups: Mahuang(Herba Ephedra Sinica), Wuweizi(Fructus Schisandrae Chinensis), and blank serum. After treatment with the herbs, serum was extracted from the rats. AM and IM were isolated from normal rats and cultured. The effects of Mahuang(Herba Ephedra Sinica) and Wuweizi(Fructus Schisandrae Chinensis) medicated serum on normal rat AM and IM chemotactic migration were determined by transwell assays. The CC chemokine receptor(CCR) 2, CCR5, voltage-gated Kvl. 3 K^+channel(Kv1. 3), and voltage-gated Kvl. 5 K^+channel(Kv1. 5) protein levels were analyzed by western blotting.RESULTS: The migration quantities of AM and IM in the Mahuang(Herba Ephedra Sinica) and Wuweizi(Fructus Schisandrae Chinensis) medicated serum groups were significantly higher than those in the blank serum group(P < 0.01). Compared with the Wuweizi(Fructus Schisandrae Chinensis) medicated serum group, the migration quantity of cultured rat AM in the Mahuang(Herba Ephedra Sinica) medicated serum group was significantly increased(P <0.01). Meanwhile, compared with the Mahuang(Herba Ephedra Sinica) medicated serum group, the migration quantity of cultured rat IM in the Wuweizi(Fructus Schisandrae Chinensis) medicated serum group was significantly increased(P < 0.01).CCR2, CCR5, Kv1. 3, and Kv1. 5 proteins were expressed on the AM cell surface, and showed significantly higher expression in the Mahuang(Herba Ephedra Sinica) medicated serum group compared with the Wuweizi(Fructus Schisandrae Chinensis)medicated serum group. In contrast, CCR5, Kv1.3,and Kv1.5 proteins were expressed on the IM cell surface, and showed significantly higher expression in the Wuweizi(Fructus Schisandrae Chinensis)medicated serum gOBJECTIVE： To observe the effects of serum containing Mahuang（Herba Ephedra Sinica） or Wuweizi（Fructus Schisandrae Chinensis） on the migration of alveolar macrophages（AM） and interstitial macrophages（IM） from normal rats, and to analyze and compare the mechanisms leading to cell migration differences.METHODS： Rats were randomly divided into three groups： Mahuang（Herba Ephedra Sinica）, Wuweizi（Fructus Schisandrae Chinensis）, and blank serum. After treatment with the herbs, serum was extracted from the rats. AM and IM were isolated from normal rats and cultured. The effects of Mahuang（Herba Ephedra Sinica） and Wuweizi（Fructus Schisandrae Chinensis） medicated serum on normal rat AM and IM chemotactic migration were determined by transwell assays. The CC chemokine receptor（CCR） 2, CCR5, voltage-gated Kvl. 3 K~＋channel（Kv1. 3）, and voltage-gated Kvl. 5 K~＋channel（Kv1. 5） protein levels were analyzed by western blotting.RESULTS： The migration quantities of AM and IM in the Mahuang（Herba Ephedra Sinica） and Wuweizi（Fructus Schisandrae Chinensis） medicated serum groups were significantly higher than those in the blank serum group（P 〈 0.01）. Compared with the Wuweizi（Fructus Schisandrae Chinensis） medicated serum group, the migration quantity of cultured rat AM in the Mahuang（Herba Ephedra Sinica） medicated serum group was significantly increased（P 〈0.01）. Meanwhile, compared with the Mahuang（Herba Ephedra Sinica） medicated serum group, the migration quantity of cultured rat IM in the Wuweizi（Fructus Schisandrae Chinensis） medicated serum group was significantly increased（P 〈 0.01）.CCR2, CCR5, Kv1. 3, and Kv1. 5 proteins were expressed on the AM cell surface, and showed significantly higher expression in the Mahuang（Herba Ephedra Sinica） medicated serum group compared with the Wuweizi（Fructus Schisandrae Chinensis）medicated serum group. In contrast, CCR5, Kv1.3,and Kv1.5 proteins were expressed on the IM cell surface,

关 键 词：Ephedra sinica Schisandra Chinensis Macrophage Receptors CCR Delayed rectifier po tassium channel Chemotaxis 

分 类 号：R285.5[医药卫生—中药学]