An essential role for PNLDC1 in piRNA 3＇ end trimming and male fertility in mice  

作　　者：Yue Zhang Rui Guo Yiqiang Cui Zhiping Zhu Yingwen Zhang Hao Wu Bo Zheng Qiuling Yue Shun Bai Wentao Zeng Xuejiang Guo Zuomin Zhou Bin Shen Ke Zheng Mingxi Liu Lan Ye Jiahao Sha 

机构地区：[1]State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, 210029, China [2]The People's Hospital of Gaochun, Nanjing, Jiangsu 210029, China

出　　处：《Cell Research》2017年第11期1392-1396,共5页细胞研究（英文版）

基　　金：This work was supported by the National Key R＆D Program of China （2016YFA0500902 and 2017YFA0103803）, the National Basic Research Program of China （2015CB943003）, National Natural Science Foundation of China Grant （81471502, 31771651, 31471228）, Excellent Youth Foundation of Jiangsu Scientific Committee（grant BK20150047）, and Innovative and Entrepreneurial Program of Jiangsu Province. We thank Jingdi Hu （Shanghai Biotechnology Corporation, Shanghai, China） for smalt RNA analysis.

摘　　要：Dear Editor, PIWI-interacting RNAs （piRNAs） are germ cell-specific small non-coding RNAs that are essential for silenc- ing transposable elements. Substantial efforts in the past decade have led to an understanding of how piRNAs are made. Primary piRNA biogenesis is initiated with transcription of piRNA precursors, followed by cleavage into piRNA intermediates, and finally, maturation by 3＇ end trimming and 2＇-O-methylation. Secondary piRNA biogenesis occurs through an amplification loop （ping pong pathway）; the piRNA pools generated through pri- mary processing guide MILI protein to cleave the target RNA for piRNA generation in a feed-forward loop that accelerates production of the piRNAs. Papi/Tdrkh has been implicated in processing the 3＇ ends of piRNAs [1, 2], however,

分 类 号：Q[生物学]