TGF-β1对子宫内膜基质细胞的增殖和活性的影响  

Effect of TGF-β1 on proliferation and activity of human endometrial stromal cells

作　　者：张文博[1] 单铁英[2] 韩文华[3] 王春漩 朱惠 杨以会[2] 潘秀兰[2] 任俊利 

机构地区：[1]邯郸市中心医院妇产科,河北邯郸056002 [2]河北工程大学医学院组胚教研室,河北邯郸056002 [3]河北工程大学附属医院内科,河北邯郸056002 [4]邯郸市中医院,河北邯郸056002 [5]武安市中医院内科,河北武安056300 [6]邯郸市中心医院儿科,河北邯郸056002

出　　处：《职业与健康》2017年第21期2939-2942,共4页Occupation and Health

摘　　要：目的观察和分析TGF-β1对子宫内膜基质细胞(endometrial stromal cells,ESCs)增殖和活性的影响。方法体外分离、培养和鉴定ESCs。实验分组:对照组和TGF-β1组,对照组:加入不含血清的培养液;转化生长因子-β1(TGF-β1)组:不含血清的培养液+不同浓度的TGF-β1,通过噻唑蓝法检测各组细胞的增殖率;用ELISA法检测各组细胞培养液中Ⅰ型胶原(Collagen typeⅠ,ColⅠ)和纤黏连蛋白(Fibronectin,FN)的含量。免疫印迹法检测各组细胞胞质中的MMP-9及TIMP-1的表达水平。结果 TGF-β1可以促进ESCs增殖,并呈剂量依赖性;使细胞分泌ColⅠ(0.736 6±0.042 4)和FN(0.713 1±0.046 8)的量增多,细胞内MMP-9含量减低,而TIMP-1含量升高;与对照组比较,其差异有统计学意义(P<0.05)。结论 TGF-β1能使子宫内膜基质细胞增殖,并使其活性增加。[Objective] To observe and explore the effect of TGF-β1 on proliferation and activity of human endometrial stromal ceils （ESCs）. [Methods] The ESCs was isolated,cultured and identified in vitro. The experiment groups include： control group and TGF-β1 groups. Control group： ESCs was cultivated by solution without serous. TGF-β1 groups： ESCs was cultivated by solution （without serous） with different concentration of TGF-β1.The method of MTF was used to detect the proliferation of ESCs. The contents of fibronectin （FN） and collagen type I （Col I ） in the cultured supernatants were measured by enzyme-linked immunosorbent assay （ELISA）. Western blot was used to detect the expression levels of MMP-9 and TIMP-1. [ Results] TGF-I31 improved the proliferation of ESCs,with a dose-dependent effect. The contents of Col I （0.736 6±0.042 4） and FN（0.713±0.046 8） increased,MMP-9 level decreased and TIMP-1 level increased,and the differences were statistically significant between control group and TGF-β1 groups （P〈O.OS）.[Conclusion] TGF-β1 can promote ESCs proliferation and increase activity of ESCs.

关 键 词：转化生长因子-Β1 子宫内膜基质细胞 金属基质蛋白酶9 金属基质蛋白酶抑制剂-1 Ⅰ型胶原 纤粘连蛋白 

分 类 号：R34[医药卫生—基础医学]