内毒素血症的犬体温及相应细胞因子动态变化观察  

Observe the Dynamic Changes of Body Temperature and Corresponding Cytokines in Dogs with Endotoxemia

作　　者：向勇平[1,2] 崔辉 刘理金[1,2] 王琳 刘丹 于杰[4] 周立冬 杨建明[5] 刘爱兵 

机构地区：[1]武警总医院医学实验中心,北京100039 [2]锦州医科大学武警总医院研究培养基地 [3]灾害救援医学北京市重点实验室 [4]天津市阳泉医疗器械有限公司 [5]天津市港口医院

出　　处：《中国急救复苏与灾害医学杂志》2017年第10期959-961,共3页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：国家高技术研究发展计划（863计划：2015AA033904）

摘　　要：目的观察注入内毒素后犬体温及血清中相应细胞因子水平动态变化。方法12只比格犬分为实验组（8只）和对照组（4只）。实验组静脉注射内毒素制造内毒素血症模型，对照组注射等量生理盐水，之后在相应的时间点测量肛温，并取血监测血清中内毒素及细胞因子的水平。结果实验组的内毒素水平在造模后24h达到峰值（306．56±10．57）EU／mL，随后下降，96h时内毒素为（3．11±1．41）EU／mL，与造模前（0．048±0．017）EU／mL相比差异有统计学意义（P〈0．05）。实验组体温上升并达峰值（40．2±0．26）℃，48h后又下降，对照组体温趋于平稳。实验组细胞因子TN-α、IL-1β、IL-6、IL-8水平在造模后开始上升，达峰值后下降。结论造模后体温和细胞因子随血液中内毒素水平的变化而变化。Objective To observe the changes of body temperature and serum eytokines in beagle dogs after endotoxin injection. Methods A total of 12 beagle dogs were divided into an experimental group （n=8） and a control group （n=4）. The experimental group was injected with endotoxin for modeling, while the control group was injected with normal saline. Then, the anal temperature was measured at the corresponding time points, and the levels of serum endotoxin and cytokines were monitored. Results The level of endotoxin in the experimental group reached the peak [（306.56±10.57） Eu/mi] 24 h after modeling and then decreased to （3.11±1.41） Eu/ml 96 h after modeling, which were statistical different from the levels before modeling （0.048 ±0.017） Eu/ml （P〈0.05）. For the experimental group, the body temperature was increased and reached the peak （40.2±0.26）℃, and then decreased 48 h after modeling and tended to be stable. The experimental group showed increases in the levels of TNF-α, IL-1β, IL-6 and IL-8 after modeling and reached the peak before decreased. Conclusions The body temperature and cytokines change after modeling as the level of endotoxin changes.

关 键 词：内毒素血症 体温 细胞因子 动态变化 

分 类 号：R631.1[医药卫生—外科学]