Effect of herbal cake-partitioned moxibustion on MEK1/2 and ERK1/2 expressions of gastric tissues in rats with spleen deficiency syndrome  

隔药饼灸对脾虚证大鼠胃组织MEK1/2及ERK1/2的影响（英文）

作　　者：贲定严 刘琼 易展 李丹 邹逸凡 马明珠 刘密 常小荣 杨燕萍 

机构地区：[1]Hunan University of Chinese Medicine

出　　处：《Journal of Acupuncture and Tuina Science》2017年第5期305-310,共6页针灸推拿医学（英文版）

基　　金：supported by National Basic Research Program of China,973 Program,No.2015CB554502;Fund Project of Hunan Province Education Office,No.13C685;Graduate Student Research Innovation Project of Hunan Province,No.CX2016B351~~

摘　　要：Objective： To observe the effect of herbal cake-partitioned moxibustion on the expressions of mitogen-activated protein kinase （MEK：1/2） and extracellular regulatory protein kinase （ERK：1/2） in gastric tissues of rats with spleen deficiency syndrome, and to explore the possible mechanisms of herbal cake-partitioned moxibustion in treating spleen deficiency syndrome. Methods： Sixty Sprague-Dawley （SD） rats were randomly divided into a blank control group （group A）, a model group （group B）, a ranitidine group （group C）, and a herbal cake-partitioned moxibustion group （group D） by random digit, 15 rats in each group. Rat models of spleen deficiency syndrome were made by intragastric administration of 4℃ 200% concentrated Da Huang （Radix et Rhizoma Rhei）. After successful modeling, the rats in group C were treated with 25 mg/（kg.bw） ranitidine by intragastric adminstration and rats in group D were treated with herbal cake-partitioned moxibustion at Zusanli （ST 36） and Zhongwan （CV 12）, for 8 d. Excepted for rats in group A, all the other rats were treated with indomethacin at 5 mg/（kg.bw） at 8：00 a.m. on the second day after finishing all the intervention and sacrificed 7 h later to isolate the stomach. Histopathological changes of the gastric tissues were observed under light microscope after hematoxylin-eosin （HE） staining. The protein expressions of MEK：1/2 and ERK：1/2 in the gastric tissues were detected by immunohistochemistry. Results： After intervention, the gastric mucosal injury in group B was significantly severer than that in group A, with large breakage and ablating; the damage of gastric mucosa was decreased in group C compared with group B; the gastric mucosal surface remained relatively complete, and the status of breakage and ablating was significantly improved. After intervention, compared with group A, the protein expressions of MEK：1/2 and ERK：12 in gastric tissues of the other groups were significantly higher （P〈0.01）.目的:通过观察隔药饼灸对脾虚证大鼠胃组织分裂原活化蛋白激酶(MEK1/2)及细胞外调节蛋白激酶(ERK1/2)蛋白表达的影响,探讨隔药饼灸治疗脾虚证的可能作用机制。方法:将60只SPF级Sprague-Dawley(SD)大鼠按随机数字表法分为空白对照组(A组)、模型组(B组)、雷尼替丁组(C组)和隔药饼灸组(D组),每组15只。通过200%的大黄浓缩液4℃灌胃制作脾虚证大鼠模型。造模成功后,C组按25 mg/(kg·bw)灌胃给药,D组接受隔药饼灸足三里和中脘治疗,连续治疗8 d。除A组外,其余组大鼠于治疗完成的第二天上午8:00接受消炎痛5 mg/(kg·bw)灌胃,7 h后处死动物,取胃。应用苏木精-伊红(HE)染色,光镜下观察大鼠胃组织病理学改变,采用免疫组化法检测大鼠胃组织MEK1/2及ERK1/2蛋白表达。结果:干预结束后,与A组相比,B组大鼠胃黏膜损伤明显,可见较大的破损、脱落;与B组比较,C组大鼠胃黏膜表面有部分脱落,破损情况改善,D组大鼠胃黏膜表面较完整,脱落及破损明显改善。干预结束后,与A组比较,其他各组大鼠胃组织MEK1/2及ERK1/2蛋白表达明显升高(均P<0.01);与B组比较,C组和D组大鼠胃组织MEK1/2及ERK1/2蛋白表达升高(均P<0.01);与C组比较,D组胃组织MEK1/2及ERK1/2蛋白表达升高(P<0.01)。结论:隔药饼灸通过提高胃组织MEK1/2及ERK1/2蛋白表达,激活MEK/ERK信号转导通路,进而促进脾虚证大鼠胃黏膜的修复。

关 键 词：Moxibustion Therapy Indirect Moxibustion Point  Zusanli （ST 36） Point  Zhongwan （CV 12） Research on Acupoints Gastric Mucosal Damage Spleen Deficiency Syndrome RATS 

分 类 号：R2-03[医药卫生—中医学]