蒙古口蘑交配型鉴定分析  

Identification for Mating Type of Tricholoma mongolicum

作　　者：刘晓婷[1] 郭九峰[1] 王淑妍[1] 孙国琴[2] 李亚娇[2] 那日[1] 

机构地区：[1]内蒙古大学物理科学与技术学院离子束生物工程自治区重点实验室,内蒙古呼和浩特010021 [2]内蒙古农牧业科学院蔬菜研究所,内蒙古呼和浩特010031

出　　处：《中国食用菌》2017年第6期18-22,共5页Edible Fungi of China

基　　金：国家自然科学基金(No.51267014)

摘　　要：对3株野生蒙古口蘑Tricholoma mongolicum Imai.子实体进行担孢子收集,采用单孢稀释法分离鉴定得到300株蒙古口蘑单孢菌丝体,利用三轮交配法对其中101株进行交配型鉴定,试验统计结果进行χ~2检验,并对4种不同交配型的单核菌株进行ISSR分子标记验证。结果显示,可观察到典型的4种交配反应菌落形态并可用ISSR分子标记技术加以区分验证。所得蒙古口蘑交配类型属于四极性交配系统,为A、B因子互不连锁的双因子控制。This study is to identificate for mating type of Tricholoma mongolicum by three rounds of mating method. We first col- lected the basidiospores of three strains of wild T. mongolicum, and 300 strains of mononuclear myceliums were obtained by sin- gle spore dilution method. Then the mating type on the 101 strains were tested and the chi-square test to the results were made. It was verified the four different mating type of mononuclear myceliums by ISSR molecular markers. The results showed that edi- ble fungi of T. mongolicum belonged to the typical tetrapolar heterothall^c mating system. It can be observed typical four mating reactions of their colony morphology and can be used ISSR molecular markers to distinguish validation.

关 键 词：蒙古口蘑 单核菌丝 三轮交配法 ISSR 交配类型 

分 类 号：S646[农业科学—蔬菜学]