紫花苜蓿苗期耐盐性鉴定  

Identification of Salt Tolerance of Alfalfa in Seedling Stage

作　　者：张喜斌[1] 秦楚[1] 麻冬梅[2] 许兴[2] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]宁夏大学农学院,宁夏银川750021

出　　处：《北方园艺》2017年第22期43-50,共8页Northern Horticulture

基　　金：宁夏自然科学基金资助项目(NZ15002)

摘　　要：以60个国内外紫花苜蓿品种为试材,通过表型鉴定的方法筛选出耐盐、敏盐品种,并利用主成分分析和隶属函数法对初步筛选出品种的叶绿素含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性及丙二醛(MDA)含量进行综合评价,根据综合评价值(D值)对耐盐性进行强弱排序,以期为紫花苜蓿遗传群体的构建及耐盐性相关基因的发掘奠定基础。结果表明:在150mmol·L^(-1) NaCl胁迫下,‘Asi’>"兴平">"秘鲁">"草原3号">国产苜蓿;在300 mmol·L^(-1) NaCl胁迫下,"阿迪娜">"巨能">"巨能7号">"抗旱15">"骑士T"。筛选出了耐盐、敏盐的种质资源材料。60 domestic and foreign alfalfa varieties were used as test materials,screening of salt tolerant and salt sensitive varieties by means of phenotypic identification,and using principal component analysis and the method of membership function of screened varieties of chlorophyll content,superoxide dismutase（SOD）,catalase（CAT）and peroxidase（POD）,malondialdehyde（MDA）comprehensive evaluation.According to the comprehensive evaluation value（D value）of salt tolerance in order to laid the foundation for the construction of the genetic groups and the related genes of salt tolerance.The results showed that in 150 mmol·L^（-1） under the stress of NaCl,‘Asi＇〉‘Xingping＇〉‘Peru＇ 〉‘Caoyuan No.3＇domestic alfalfa;in 300 mmol· L^（-1） under NaCl stress,‘Adina＇〉‘Juneng＇〉‘Juneng No.7＇〉‘Kanghan 15＇〉‘Qishi T＇.The germplasm resources of salt tolerance and salt tolerance were screened.

关 键 词：紫花苜蓿 水培 苗期 耐盐性 

分 类 号：S551.7[农业科学—作物学]