闲置厌氧氨氧化微生物的启动及微生物分析  

Nitrogen Removal Performance of Anammox Biomass during the Restarting after a Long-term Abandoning and Microbial Community Analysis

作　　者：高岩 周北海 孙晨[2,3] 田智勇[2,3] 

机构地区：[1]北京科技大学能源与环境工程学院,北京100083 [2]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [3]中国环境科学研究院城市水环境科技创新基地,北京100012

出　　处：《环境保护科学》2017年第5期43-50,共8页Environmental Protection Science

基　　金：国家科技重大水专项课题(2013ZX07202-010)基金资助

摘　　要：文章以闲置12个月的厌氧氨氧化生物膜填料重新启动厌氧氨氧化反应器,并对反应器的活性恢复情况、脱氮效果和微生物菌群结构开展研究。实验结果表明:在启动的200 d逐渐将进水氨氮、亚硝态氮浓度从50 mg/L提高到70 mg/L,水力停留时间从12 h缩短到4 h,后期氨氮去除率达80%以上,亚硝酸盐去除率达95%。170~200 d的稳定期中,平均去除负荷0.71±0.15 kg/(m^3·d)。另外,通过高通量测序技术对反应器中微生物群落变化情况进行了系统分析。启动过程中填料中污泥微生物浮霉菌门Planctomyctes的相对丰度从13.7%增长到了36.0%,成为优势菌群。In this study, an anammox reactor was restarted up with used anammox biofilm stuffing which had lay idle for 12 months. Recovery of the biological activity, nitrogen removal and microbial community structure of the reactor were investigated. During 200 days of the restart-up period, concentrations of NH4＋-N and NO2--N increased from 50 mg/L to 70 mg/L, and hydraulic retention time （HRT） was shortened from 12 h to 4 h gradually. The results indicated that the removal rate of NH4＋-N and NO：--N in the later period reached to over 80% and 95% respectively. During the stable period between 170 and 200 d, the average nitrogen removal load was 0.71 ± 0.15 kg/（m3· d）. In addition, high-throughput sequencing technology was employed to analyze the change of microbial communities during the restart-up. Relative abundance of Planctomyctes species on the packing biofilms increased from 13.7% to 36.0% and therefore the species became the dominant.

关 键 词：厌氧氨氧化 生物膜填料 高通量测序 

分 类 号：X703.1[环境科学与工程—环境工程]