基于寄主差异的桑寄生中广寄生苷、槲皮苷、槲皮素含量分析  

Content analysis of avicularin,quercitrin and quercetin in Herba Taxilli from different host plants

作　　者：苏本伟 王辉 李永华[3] 裴河欢 朱开昕 卢栋 

机构地区：[1]钦州市中医药研究所,钦州535099 [2]钦州市中医药研究所,钦州535099 [3]广西中医药大学,南宁530001 [4]钦州市妇幼保健院,钦州535099

出　　处：《国际药学研究杂志》2017年第7期738-742,共5页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金面上资助项目(81173537);广西自然科学基金资助项目(2015GXNSFBA139132);广西中医药科技专项资助项目(GZBZ14-21、GZBZ16-17)

摘　　要：目的研究不同寄主来源桑寄生中广寄生苷、槲皮苷、槲皮素3种活性成分含量的差异。方法采用双波长切换法测定来源于不同寄主植物桑寄生样品中广寄生苷、槲皮苷和槲皮素含量,样品采用甲醇超声提取方法制备。色谱条件:Ultimate XB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-甲醇(B)-0.1%磷酸溶液(C),梯度洗脱,流速:1.0 ml/min,柱温:25℃,双波长切换检测。结果广寄生苷、槲皮苷、槲皮素的线性范围分别为0.0992~1.9840(r=0.9999)、0.2254~4.4580(r=0.9999)和0.1258~2.5160μg(r=0.9997),平均加样回收率(n=5)分别为98.39%、97.08%和98.15%。不同寄主来源桑寄生药材茎枝中广寄生苷、槲皮苷、槲皮素含量分别为0.0000~0.0398、0.3977~0.7639和0.0068~0.0231 mg/g,叶中为0.0167~0.1704、1.8626~11.0041和0.0185~0.1841 mg/g。结论该法准确度高、简便快捷、重现性好,可作为桑寄生药材的质量控制标准。Objective To study the content difference of avicularin,quercitrin and quercetin in Herba Taxilli from different host plants. Methods The contents of avicularin,quercitrin and quercetin in Herba Taxilli were determined by RP-HPLC and the samples were prepared by ultrasonic extraction with methanol. The analytical column was UltImate XB-C18（250 mm＆#215;4.6 mm,5μm）, the mobile phase was acetonitrile-methanol-0.1%phosphate with gradient elution,at a flow rate of 1.0 ml/min. The column tempera-ture was set at 25℃,the detection wavelength was 254 nm for avicularin and quercitrin,and 365 nm for quercetin. Results The lin-ear range of the above three ingrediets were 0.0992-1.9840（r=0.9999）,0.2254-4.4580（r=0.9999）and 0.1258-2.5160μg（r=0.9997） and the average recovery rates（n=5）were 98.39%,97.08%and 98.159%,respectively. Their contents from different host plants were 0.0000-0.0398,0.3977-0.7639 and 0.0068-0.0231 mg/g in branches and 0.0167-0.1704,1.8626-11.0041,and 0.0185-0.1841 mg/g in leaves. Conclusion The method is accurate,simple and reproducible,and can be used for the quality control of Herba Taxilli.

关 键 词：桑寄生 寄主差异 广寄生苷 槲皮苷 槲皮素 

分 类 号：R284.1[医药卫生—中药学]