输血相关急性肺损伤大鼠肺组织核转录因子-κB和细胞间黏附分子-1表达及其意义  

Change in expression of nuclear factor-κB and intercellular adhesion molecule-1 in lung tissues of rats with transfusion-related acute lung injury and its significance

作　　者：涂玲[1] 魏明[1] 刘佳[1] 梁颖红[1] 张宜花[1] 陈河涛 杨璐[1] 肖笛鸣 

机构地区：[1]郑州大学第五附属医院输血科,450052

出　　处：《中华生物医学工程杂志》2017年第3期208-214,共7页Chinese Journal of Biomedical Engineering

摘　　要：目的 研究输血相关急性肺损伤（TRALI）大鼠肺组织核转录因子（NF）-κB及细胞间黏附分子（ICAM）-1表达变化,并探讨其变化的临床意义.方法 60只SD大鼠按随机数字表法分为正常对照组、脂多糖对照组、TRALI组和阳性对照组各15只.正常对照组：采用假手术处理;脂多糖对照组：经大鼠腹腔注射脂多糖（2 mg/kg,≤1 min完毕）,2 h后静脉输注生理盐水（约1 ml/只）;TRALI组：腹腔注射脂多糖（2 mg/kg）2 h后静脉输注血浆（约1 ml/只）;阳性对照组：静脉输注脂多糖（5 mg/kg）诱导急性肺损伤.支气管肺泡灌洗液（BALF）沉渣行瑞士染色进行白细胞分类计数;大鼠肺组织病变进行半定量肺损伤评分;采用免疫组织化学法对肺泡上皮细胞中ICAM-1表达进行定位及半定量分析,并对NF-κB核转位进行半定量分析.结果 阳性对照组和TRALI组大鼠支气管肺泡灌洗液多形核白细胞（PMN）比例[（39.6±12.5）％和（33.5±11.5）％]及肺损伤评分[（2.32±0.70）和（1.98±0.87）]均明显高于正常对照组[（9.8±3.5）％和（0.00±0.00）]和脂多糖对照组[（11.4±4.1）％和（1.07±0.48）],（均P〈0.05）;阳性对照组和TRALI组大鼠肺泡上皮细胞中ICAM-1蛋白[（0.28±0.09）和（0.24±0.07）]和ICAM-1 mRNA表达[（1.53±0.17）和（1.24±0.11）]明显高于正常对照组[（0.12±0.03）和（0.36±0.05）]和脂多糖对照组[（0.16±0.07）和（0.89±0.10）]（均P〈0.05）;阳性对照组和TRALI组大鼠肺泡上皮细胞中NF-κB核表达[（23.8±3.20）和（27.4±4.60）]明显高于正常对照组（6.90±2.30）和脂多糖对照组（11.7±2.80）,（均P〈0.05）.结论 TRALI大鼠肺组织中ICAM-1蛋白和ICAM-1 mRNA的表达升高,肺组织PMN浸润增加,同时大鼠肺泡上皮细胞中NF-κB核表达升高,提示ICAM-1可能参与了PMN与内皮细胞的黏附和聚集,推测NF-κB激活可能参与了TRALI炎症介质ICAM-1基因转录调控,在TRALI的病�Objective To determine the change in the expression of nuclear factor-κB（NF-κB） and intercellular adhesion molecule 1（ICAM-1）in the lung tissues of rats with transfusion-related acute lung injury,and to investigate its clinical significance. Methods Sixty SD rats were randomly divided into the normal control group,lipopolysaccharide（LPS）control group,TRALI group and positive control group（n=15 each）. The normal control group was treated with sham operation. The LPS control group was intraperitoneally injected with LPS （2 mg/kg,completed within ≤ 1 min）,and was given intravenous infusion of normal saline at 2 h（about 1 ml/rat）. The TRALI group was treated with intravenous infusion of blood（about 1 ml/rat）at 2 h after intraperitoneal injection of LPS（2 mg/kg）. The positive control group was treated with intravenous infusion of LPS（5 mg/kg）to induce acute lung injury. The sediment of bronchial alveolar lavage fluid（BALF）was determined by Wright-Giemsa staining for differential leukocyte count. The lesions in the lung tissue of rats were assessed by semi-quantitative lung injury scoring. The expression of ICAM-1 in alveolar epithelial cells was determined by immunohistochemistry and semi-quantitative analysis. The NF-κB nuclear translocation was determined by semi-quantitative analysis. Results In the positive control group and the TRALI group,the ratio of polymorphonuclear leukocytes（PMN）in the bronchoalveolar lavage fluid[（39.6±12.5）%and（33.5±11.5）%]and the lung injury score[（2.32±0.70）and（1.98±0.87）] were significantly higher than those in the normal control group[（9.8±3.5）%and（0.00±0.00）]and the LPS control group[（11.4±4.1）%and（1.07±0.48）],respectively（all P〈0.05）. In the positive control group and TRALI group,the expressions of ICAM-1 protein[（0.28 ± 0.09）and（0.24 ± 0.07）]and ICAM-1 mRNA [（1.53 ± 0.17）and（1.24 ± 0.11）]in the alveolar epithelial cells were significantly higher than those in the norma

关 键 词：急性肺损伤 输血相关 肺 核因子-κB 细胞间黏附分子 

分 类 号：R457.1[医药卫生—治疗学]