规模化猪场废水典型抗生素抗性基因的调查研究  

A Study on Typical Antibiotic Resistance Genes in Largescale Pig Farm Wastewater

作　　者：刘锐[1] 

机构地区：[1]嘉兴职业技术学院,浙江嘉兴314036

出　　处：《家畜生态学报》2017年第11期68-71,共4页Journal of Domestic Animal Ecology

基　　金："十二五"国家科技支撑计划项目(2013BAD21B04)

摘　　要：为了解嘉兴市规模化猪场废水抗生素抗性基因污染现状,采用荧光定量PCR技术对嘉兴市10家规模化猪场废水中tetA、tetC、tet M、tetO、tetQ、tet W、sul1和sul2等8种典型抗生素抗性基因进行定量分析。结果表明,10家规模化猪场废水中均检测到上述8种抗生素抗性基因,tetA、tetC、tet M、tetO、tetQ、tet W、sul1和sul2在废水中绝对含量分别在2.1×10~5～7.6×10~7,9.5×10~3～2.4×10~6,2.9×10~5～2.0×10~8,9.4×10~5～8.7×10~7,6.3×10~6～5.0×10~8,1.1×10~6～5.0×10~9,5.9×10~6～2.2×10~8,1.4×10~6～7.1×10~8 copies/mL之间。由此表明,规模化猪场可能是嘉兴市抗生素抗性基因污染的主要来源之一。Fluorescence quantitative PCR was applied to quantify eight typical antibiotic resistance genes （ARGs） including tetA, tetC, tetM, tetO, tetQ, tetW, sul1 and sul2 in wastewaters from ten typical largescale pig farms in Jiaxing city.The results showed that all of the eight ARGs were detectible in the wastewater from the ten largescale pig farms. The absolute content was 2.1×10^5~7.6×10^7 copies/mL of tetA, 9.5×10^3~2.4×10^6 copies/mL of tetC, 2.9×10^5~2.0×10^8 copies/mL of tetM, 9.4×10^5~8.7×10^7 copies/mL of tetO, 6.3×10^6~5.0×10^8 copies/mL of tetQ, 1.1×10^6~5.0×10^9 copies/mL of tetW, 5.9×10^6~2.2×10^8 copies/mL of sul1 and 1.4×10^6~7.1×10^8 copies/mL of sul2, respectively. The above results indicated that largescale pig farm is one of the principal contributors to ARGs pollution in Jiaxing.

关 键 词：猪场废水 四环素类抗生素抗性基因 磺胺类抗生素抗性基因 

分 类 号：S811.5[农业科学—畜牧学]