美洲大蠊提取物Ento-A对噁唑酮致大鼠溃疡性结肠炎的治疗作用研究  

Study on the Therapeutic Effect of Periplaneta americana Extract Ento-A on Oxazolone Induced Ulcerative Colitis in Rats

作　　者：张汉超 张成桂 耿福能 沈咏梅 刘彬 赵昱 刘衡 

机构地区：[1]云南省昆虫生物医药研发重点实验室,云南大理671000 [2]药用特种昆虫开发国家地方联合工程研究中心,云南大理671000 [3]中国西南药用昆虫及蛛形类资源开发利用协同创新中心,云南大理671000 [4]四川好医生药业集团,四川成都610000

出　　处：《中药材》2017年第6期1420-1424,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81660605;81260676);药用特种昆虫开发国家地方联合工程研究中心创新能力建设项目(云发改高技[2015]395);云南省昆虫生物医药研发重点实验室专项经费(2015DG030);云南省2011协同创新中心项目([2012]25);云南省应用基础研究重点项目(2014FA007)

摘　　要：目的:研究美洲大蠊提取物Ento-A对噁唑酮(OXZ)诱导大鼠溃疡性结肠炎(UC)的疗效,并初步探讨其作用机制。方法:将雄性SD大鼠分为正常对照组、模型组、美沙拉嗪组和美洲大蠊提取物Ento-A低、中、高剂量组,除正常对照组外,其余各组以噁唑酮溶液诱导大鼠溃疡性结肠炎模型。测定疾病活动指数(DAI)、脏器指数、结肠黏膜损伤指数(CMDI)和病理组织学评分(HS),检测血清IL-4、IL-10、iNOS及结肠黏膜中MPO、EGF水平,评价药物对UC大鼠的疗效。结果:与正常对照组比较,模型组血清IL-4、IL-10及结肠组织EGF水平显著降低,CMDI评分、HS评分、血清iNOS、结肠指数及结肠MPO活性显著升高。美沙拉嗪组及Ento-A各剂量组上述指标均得到不同程度改善。结论:美洲大蠊提取物Ento-A灌肠能有效缓解噁唑酮诱导的大鼠UC,其作用机制可能与下调iNOS、MPO水平,上调IL-4、IL-10及EGF水平有关。Objective：To study the therapeutic effect of Periplaneta americana extract Ento-A on oxazolone induced ulcerative colitis in rats,and to explore its mechanism.Methods：Male SD rats were divided into normal control group,model group,the mesalazine group and Ento-A low-,medium-and high-dose groups.In addition to the normal control group,other groups were induced into ulcerative colitis with oxazolone solution.The disease activity index（DAI）,organ index,colon mucosa damage index （CMDI）and histopathological score（HS）,the levels of IL-4,IL-10,iNOS in serum,and MPO and EGF in colonic mucosa were dected to evaluate the therapeutic effect of drug on ulcerative colitis in rats.Results：Compared with the normal group,the level of IL-4 and IL-10 in serum,EGF in colonic were reduced significantly,while the CMDI score,HS score,the content of serum iNOS,colon index and MPO in colonic were increased significantly in the model group.Abore indicators of mesalazine group and Ento-A low-,medium-and high-dose groups were with varying degrees of improvement.Conclusion：Periplaneta americana extract Ento-A can effectively alleviate oxazolone induced ulcerative colitis in rats,the mechanism may be related to the down regulation of iNOS,MPO levels and the up regulation of IL-4,IL-10,EGF levels.

关 键 词：溃疡性结肠炎 美洲大蠊提取物Ento-A 噁唑酮 作用机制 

分 类 号：R285.5[医药卫生—中药学]