冠心病患者ApoE基因多态性及其脂质谱相关性研究  

Relationship between ApoE Gene Polymorphism and Lipid Spectrum in Patients with Coronary Heart Disease

作　　者：吕小林[1] 万腊根[1] 刘淑媛[1] 

机构地区：[1]南昌大学第一附属医院检验科,南昌330006

出　　处：《南昌大学学报（医学版）》2017年第5期20-22,40,107,共5页Journal of Nanchang University：Medical Sciences

摘　　要：目的研究冠状动脉粥样硬化性心脏病(冠心病,CHD)患者的载脂蛋白E(ApoE)基因多态性及其与血清脂质水平之间的关系。方法采用生物化学方法检测401例CHD(CHD组)的总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL)、高密度脂蛋白胆固醇(HDL)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)、脂蛋白a(LPa),应用基因芯片法检测CHD组患者及对照组(60例同期健康者)的ApoE多态性。结果 CHD组共检出6种基因型,分别为E2/E2、E3/E3、E4/E4、E3/E4、E2/E3、E2/E4,对照组检出E3/E3、E3/E4、E2/E3、E2/E4,与对照组相比,CHD组e4等位基因频率增高(P<0.05);CHD组中不同基因型其血脂水平存在一定差异,与E3/E3相比,E2/E2基因型TC、Lpa水平较低,HDL、ApoA水平较高,E4/E4基因型TC、LDL、Lpa水平较高(P<0.05)。结论ApoE多态性与机体脂质代谢密切相关,e4等位基因可能是CHD发病重要遗传因素之一。Objective To study the relationship between apolipoprotein E（ApoE）gene poly-morphism and serum lipid levels in patients with coronary heart disease（CHD）.Methods Levels of total cholesterol（TC）,glycerin（TG）,low density lipoprotein cholesterol（LDL）,high density lipoprotein cholesterol（HDL）,apolipoprotein A1（ApoA1）,apolipoprotein B（ApoB）and lipopro-tein a（LPa）were detected using biochemical methods in 401 patients with CHD.In addition, ApoE gene polymorphism was measured using gene-chip method in CHD patients and 60 healthy controls.Results Six genotypes（E2/E2,E3/E3,E4/E4,E3/E4,E2/E3,E2/E4）were found in CHD group,and 4 genotypes（E3/E3,E3/E4,E2/E3,E2/E4）in control group.Compared with control group,the frequency of e4 increased in CHD group（P 〈0.05）.Compared with CHD pa-tients with E3/E3 genotype,levels of TC and Lpa decreased and levels of HDL and ApoA in-creased in patients with E2/E2 genotype,and levels of TC,LDL and Lpa increased in patients with E4/E4 genotype（P 〈0.05）.Conclusion ApoE gene polymorphism is closely related to lipid metabolism.The e4 gene may be one of the important genetic factors for CHD.

关 键 词：载脂蛋白 基因多态性 冠状动脉粥样硬化性心脏病 血脂 

分 类 号：R541.4[医药卫生—心血管疾病]