扭脱甲基杆菌AM1生物加工过程研究进展  

Progress in bioprocessing of value-added methanol product

作　　者：王山山 崔兰玉 薛正莲 张翀[2,3] 邢新会[2,3] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000 [2]清华大学化学工程系生物化工研究所工业生物催化教育部重点实验室,北京100084 [3]清华大学合成与系统生物学中心,北京100084

出　　处：《生物加工过程》2017年第6期26-33,共8页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21376137)

摘　　要：甲醇作为重要的一碳化工原料,可由甲烷、合成气和CO_2等转化制得。扭脱甲基杆菌AM1是以甲醇作为唯一碳源和能源来进行生物发酵的微生物。由于原料来源广泛,发酵过程染菌的风险低,且能够利用合成培养基降低生产成本,同时产物后处理简单,扭脱甲基杆菌AM1被广泛利用在生物化工领域。目前,扭脱甲基杆菌AM1以甲醇作为碳源,已经成功实现了工业化生产单细胞蛋白。随着分子生物学技术的发展,扭脱甲基杆菌AM1以甲醇为底物有望合成更多高附加值的产品。本文中,笔者对扭脱甲基杆菌AM1生物加工过程进行综述,希望对甲醇基生物工业有所启发。Methanol as an important feedstock for one？carbon industry,can be produced from methane,syngas and carbon dioxide.Because of good availability of raw materials,low risk of contamination duringthe fermentation process,and compatibility with synthetic media,bio？fermentation with methanol as carbonsource can significantly reduce production cost and simplify product down？streamprocessing, henceMethylobacterium extorquens AM1 has been widely applied in biochemical engineering. For example,fermentation with methanol as carbon source,M. extorquens AM1 has been demonstrated as a successfulapproach to achieve the industrialization of single？cell protein production. With the development ofmolecular biology methods,microbial cell factory with methanol as the feedstock is expected to expand itsvalue？added product spectrum. In this article, we reviewed the recent advances in bioprocessing ofmethanol？based value？added product by M. extorquens AM1 to enlighten the methanol？based bioindustry.

关 键 词：甲醇 生物化工 高附加值产品 扭脱甲基杆菌AM1 

分 类 号：Q93[生物学—微生物学]