生物素化DNA与链霉亲和素绑定初步研究  

Preliminary Studies on the Binding of Biotinylated DNA to Streptavidin

作　　者：杨秋萍[1] 何珊珊[1] 王志民[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2016年第6期9-13,20,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海交通大学创新基金支持项目(X198775)

摘　　要：将生物素标记的DNA与链霉亲和素(streptavidin,STV)按4∶1摩尔比连接,用原子力显微镜(atomic force microscope,AFM)对混合物表征,观察到所有可能的几种DNA-STV复合物。并按照不同摩尔浓度比和孵育时间配制DNA与STV混合液,探讨了影响结合效率的因素,初步得到用生物素-链霉亲和素系统批量制备1STV-1DNA单分子复合物的最佳制备条件,为未来单分子DNA的大规模快速制备奠定了基础。Mixture conjugation products between biotinylated DNA and streptavidin(STV)at a molar ratio of 4:1were characterized using atomic force microscope(AFM),and a few but not all types of DNA-STV conjugates were observed.Meanwhile,conjugation efficiency was investigated under various DNA to STV ratios and incubation time.The optimum parameters to produce 1STV-1DNA conjugate were preliminary obtained,providing a foundation for facile and large-scale preparation of single molecule DNA conjugate.

关 键 词：生物素-链霉亲和素系统 DNA-STV复合物 AFM 1STV-1DNA单分子复合物 

分 类 号：Q78[生物学—分子生物学]