青蒿非分泌型腺毛特异TPS7基因启动子的克隆及功能分析  

Cloning and Analysis of the Promoter of a Non-Glandular Trichome Specific Gene TPS7 in Artemisia annua L.

作　　者：陈俏[1] 付雪晴[1] 颜廷祥[1] 石璞[1] 郝小龙[1] 唐克轩[1] 

机构地区：[1]上海交通大学农业与生物学院,交大-复旦-诺丁汉植物生物技术研发中心,上海200240

出　　处：《上海交通大学学报（农业科学版）》2016年第6期21-28,共8页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家836计划(2011AA100605)

摘　　要：研究运用基因组步移法克隆了长度为1 167bp的青蒿启动子proTPS7,经生物信息学分析发现了proTPS7中的多个顺式调控元件。构建启动子与GUS融合载体,稳定转化青蒿并对转基因植株进行GUS染色实验,结果表明该启动子能驱动报告基因在非分泌型腺毛中的特异表达。对转基因青蒿喷洒甲基茉莉酸和脱落酸,3h后GUS基因表达水平有所上调,说明proTPS7能受到上述2种激素的诱导。A promoter of 1 167 bp called proTPS7 was isolated from Artemisia annua L.by genomic walking.Several cis-acting elements were discovered via bioinformatics.Then it was fused to GUSreporter gene and transformed into A.annua.GUS staining assay showed that the promoter initiated non-glandular trichome specific expression of GUS gene.GUSexpression level in transgenic A.annua was increased 3hours after MeJA and ABA treatments,indicating that proTPS7 was hormone-responsive.

关 键 词：青蒿 萜类合成酶 非分泌型腺毛 GUS染色 顺式调控元件 

分 类 号：Q943.2[生物学—植物学]