Hoechst-naphthalimide dyad with dual emissions as specific and ratiometric sensor for nucleus DNA damage  

作　　者：Fu Yang Chao Wang Lu Wang Zhi-Wei Ye Xin-Bo Song Yi Xiao 

机构地区：[1]State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China

出　　处：《Chinese Chemical Letters》2017年第10期2019-2022,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 21376038, 21421005 and 21576040);National Basic Research Program of China (No. 2013CB733702)

摘　　要：A ratiometric fluorescent sensor（Hoe-NI） was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via ＂click chemistry＂. The sensor achieves high specific nucleus labeling with wash-free staining method in various kinds of living cells. The fluorescence ratio of the two emission bands（450 nm for Hoechst and 505 nm for naphthalimide） is changed sensitively to the variation of DNA concentrations, which provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug. Therefore, Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells.A ratiometric fluorescent sensor（Hoe-NI） was developed by connecting a nucleus targeted Hoechst unit to a naphthalimide dye via ＂click chemistry＂. The sensor achieves high specific nucleus labeling with wash-free staining method in various kinds of living cells. The fluorescence ratio of the two emission bands（450 nm for Hoechst and 505 nm for naphthalimide） is changed sensitively to the variation of DNA concentrations, which provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug. Therefore, Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells.

关 键 词：DNA damage Ratiometric sensor Fluorescenct imaging Nucleus labeling NAPHTHALIMIDE 

分 类 号：O657.3[理学—分析化学] TP212.3[理学—化学]