猪乙型脑炎病毒GZ0409-31分离株E基因遗传进化分析  

Genetic Evolution of E gene of Swine Japanese Encephalitis Virus GZ0409-31 Strain

作　　者：廖洁丹 刘薇[2,3] 朱梦娇[2] 赵明秋 陈金顶 

机构地区：[1]佛山科学技术学院生命科学与工程学院,广东佛山528231 [2]华南农业大学兽医学院,广东广州510642 [3]广东永顺生物制药股份有限公司,广东广州511356

出　　处：《中国兽医杂志》2017年第9期3-5,8,共4页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划项目(2017YFD0500600;2017YF D050114);广东省科技计划项目(2015B020230009;2015A050502044)

摘　　要：本研究采用RT-PCR方法对分离自贵州省发病猪睾丸组织中的流行性乙型脑炎病毒GZ0409-31株的E基因进行克隆和序列测定,利用生物信息学软件对E基因核苷酸序列和推导的氨基酸序列进行分子遗传进化关系分析。结果表明,乙型脑炎病毒GZ0409-31毒株与基因Ⅲ型的乙脑病毒毒株属同一分支,遗传进化关系较近。In this study,the E gene of Japanese encephalitis virus GZ0409-31 strain isolated from testicular tissue of Guizhou Province was cloned and sequenced by RT-PCR. The molecular genetic evolution of E gene nucleotide sequence and deduced amino acid sequence was analyzed by bioinformatics software. The results showed that the Japanese encephalitis virus GZ0409-31 strain was the same genotype as the genotype Ⅲ of the Japanese encephalitis virus,and the genetic evolution was similar.

关 键 词：猪 乙型脑炎病毒 E基因 遗传进化分析 

分 类 号：S855.3[农业科学—临床兽医学]