细胞传代致弱的禽偏肺病毒JC毒株致病性变化的研究  

Pathogenicity Changes of Cell Attenuated Avian Metapneumovirus JC Strain

作　　者：韦莉[1] 王菁[1] 朱珊珊[1] 阎旭[1] 全荣[1] 李子璇[1] 侯磊[1] 齐宾宾 姜海军 刘爵[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所畜禽疫病防控技术北京市重点实验室,北京海淀100097

出　　处：《中国兽医杂志》2017年第9期6-8,I0001,共4页Chinese Journal of Veterinary Medicine

基　　金：北京市科技计划项目(Z141100002314013);国家现代农业产业技术体系肉鸡产业技术体系(CARS-42)

摘　　要：将禽偏肺病毒(aMPV)JC株在Vero细胞上传代传至第50代时,细胞适应毒株毒价为105.6TCID50/m L;雏鸡致病性试验结果显示,aMPV JC株细胞传代第50代与亲本毒F0相比感染雏鸡发病率降低,病理变化减轻,表明第F50细胞适应毒的毒力减弱。Avian metapneumovirus（ a MPV） JC strain was propagated in Vero cells. The virus titer reached to 105. 6 TCID5 0/m L after 50 passages. Compared to parental virus,the morbidity rate decreased and the pathological changes were alleviated in chicken after being challenged with passage 50 virus. It indicated that the virulence of F50 cell adapted strain was attenuated.

关 键 词：禽偏肺病毒JC株 细胞适应病毒 致病性 

分 类 号：S852.65[农业科学—基础兽医学]