牛源多杀性巴氏杆菌荚膜血清型及毒力基因检测  

Capsular Serotype and Virulence Gene Detection on Bovine Source Pasteurella multocida

作　　者：王羽[1] 董文龙[1] 王巍[1] 张喜庆[1] 田佳琪[1] 马红霞[1] 高云航[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国兽医杂志》2017年第9期16-19,共4页Chinese Journal of Veterinary Medicine

摘　　要：为了确定12株牛源多杀性巴氏杆菌的血清型及毒力基因的携带情况,本研究采用PCR技术对12株牛源多杀性巴氏杆菌(pasteurella maltocida,Pm)进行荚膜血清型鉴定和6种毒力基因(tbp A、hgb A、hgb B、ptf A、pfh A、tox A)的检测,并对其序列进行比对分析。结果显示:12株多杀性巴氏杆菌均为荚膜血清A型;100%的荚膜血清A型Pm携带hgb A毒力基因和pfh A毒力基因;41.67%的荚膜血清A型Pm携带nan H毒力基因;58.33%的荚膜血清A型Pm携带ptf A毒力基因;而对于tbp A与tox A两种毒力基因在12株牛源荚膜血清A型Pm中均未检测到。To determine the serotype and virulence genes of 12 strains of Pasteurella multocida. We use PCR technology for detection and sequence analysis of 12 Bovine source Pasteurella multocida（ Pm） of capsular serotype and 6 virulence genes（ ptf A,pfh A,hgb A,hgb B,tbp A,tox A）. The results showed that all 12 Pm strains are capsular serotype A,100% of the bovine source capsular serotype A Pm strains carry virulence genes pfh A and virulence genes hgb A; 41. 67% of bovine source capsular serotype A Pm strains carry virulence genes nan H; 58. 33% of bovine source capsular serotype A Pm strains carry virulence genes ptf A; tbp A and tox A was not detected in 12 bovine source capsular serotype A Pm strains.

关 键 词：多杀性巴氏杆菌 荚膜血清型 毒力基因 

分 类 号：S852.653[农业科学—基础兽医学]