肝癌细胞内HAb18G/CD147信号转导通路关键分子的筛选  

Screening of key molecules of HAb18G/CD147 signal transduction pathway in human hepatoma cells

作　　者：周宏伟[1] 姚毅冰 梁耀杰 王志红[1] 周晓燕[1] 郭卫平[2] 杨向民[3] 蒋建利[3] 孙君重[1] 陈志南[3] 

机构地区：[1]解放军总医院第一附属医院老年肿瘤科,北京100048 [2]解放军第四军医大学唐都医院介入放射科,陕西西安710038 [3]解放军第四军医大学细胞工程研究中心国家肿瘤生物学重点实验室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2017年第9期1196-1200,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30200144)

摘　　要：目的筛选肝癌细胞内HAb18G/CD147信号转导通路中差异表达的关键分子。方法提取SMMC-7721细胞和T7721细胞(SMMC-7721经稳定转染并高表达HAb18G/CD147的肝癌细胞),应用信号转导相关基因芯片筛选两种肝癌细胞内差异表达的关键分子。结果基因芯片结果显示共有13个差异表达的基因,在高表达HAb18G/CD147的T7721细胞系中,表达下调的基因包括:骨形成蛋白(BMP)家族的BMP-2、BMP-5,内皮素1(ET-1),结缔组织生长因子-富含半胱氨酸血管生成诱导物61-肾母细胞瘤过表达基因(CCN)蛋白家族的WISP-2(Wnt1-induced signaling proteins-2/CCN5)和富含半胱氨酸蛋白61(cysteine-rich 61/CCN1),前列腺干细胞抗原(PSCA);表达上调的基因包括:白细胞介素10受体α(IL-10Rα),趋化因子家族的IL-6、IL-8和CXC趋化因子配体2(CXCL2/GROβ),线粒体超氧化物歧化酶2(SOD2),B因子(B factor)和转化生长因子β(TGF-β)诱导基因克隆3(βig-h3)的表达。结论共筛选出HAb18G/CD147信号转导通路相关的13个差异表达基因,这些基因与肝癌细胞免疫微环境重塑、血管形成、增殖、侵袭和迁移等生物学过程相关。Objective To screen the differentially expressed key molecules of HAb18 G/CD147 signal transduction pathway in human hepatoma cells. Methods The total RNA was extracted from SMMC-7721 and T7721 cells,which were stably transfected and overexpressed HAb18 G/CD147,and then detected by signal transduction-related microarray to identify differentially expressed key molecules. Results The microarray data indicated that there were 13 differentially expressed genes between T7721 and SMMC-7721 cells. In T7721 cell line which overexpressed HAb18 G/CD147,the down-regulated genes included bone morphogenetic protein-2（ BMP-2）,BMP-5,endothelin-1（ ET-1）,Wnt1-induced signaling proteins-2/CCN5（ WISP-2）,cysteine-rich 61/CCN1（ Cyr61）,prostate stem cell antigen（ PSCA）,and the up-regulated genes included interleukin-10 receptor α（ IL-10 Rα）,IL-6,IL-8,CXCL2,mitochondrial superoxide dismutase 2（ SOD2）,B factor andβig-h3. Conclusion The study identified totally 13 differentially expressed genes which were related to HAb18 G/CD147 signal transduction pathway. These genes are involved in the regulation of various hepatoma biological processes,such as immune microenvironment remolding,angiogenesis,cell proliferation,invasion and metastasis.

关 键 词：HAB18G/CD147 肝癌 信号转导 骨形成蛋白2(BMP-2) TGF-β诱导基因克隆3(βig-h3) 

分 类 号：R392[医药卫生—免疫学] Q279[医药卫生—基础医学]