维替泊芬通过下调Yes相关蛋白表达抑制MDA-MB-231乳腺癌细胞增殖及侵袭和迁移  

Verteporfin inhibits proliferation,invasion and migration of MDA-MB-231 human breast cancer cells by down-regulating the expression of Yes-associated protein

作　　者：蒋玉林[1] 刘一锋[1] 张志乾[1] 杨俊鸿[1] 叶相森 靳倩妮 陈婷梅[1] 

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室,重庆400016

出　　处：《细胞与分子免疫学杂志》2017年第9期1223-1227,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81272544)

摘　　要：目的探讨维替泊芬对MDA-MB-231乳腺癌细胞增殖、侵袭和迁移的影响及其机制。方法选取对数生长期的MDA-MB-231细胞,随机分为对照组和维替泊芬处理组。先采用CCK-8法确定维替泊芬对MDA-MB-231细胞增殖抑制的最低抑制浓度,而后采用4μmol/m L维替泊芬处理MDA-MB-231细胞,采用TranswellTM侵袭实验检测细胞的侵袭能力,采用划痕实验检测细胞迁移能力;采用Western blot法检测(0、4、8、12、16)μmol/m L维替泊芬对MDA-MB-231细胞中增殖相关蛋白c-MYC、细胞周期蛋白D1(cyclin D1)、Yes相关蛋白(YAP)及其靶基因富半胱氨酸蛋白61(CYR61)和结缔组织生长因子(CTGF)的蛋白表达水平。结果维替泊芬明显抑制MDA-MB-231细胞的增殖,且呈剂量依赖性,最低抑制浓度为4μmol/m L。4μmol/m L维替泊芬显著抑制MDA-MB-231细胞的侵袭及迁移。维替泊芬下调MDA-MB-231细胞中增殖相关蛋白c-MYC、细胞周期蛋白cyclin D1及YAP、CYR61和CTGF的蛋白表达水平。结论维替泊芬通过下调YAP及其靶基因CYR61、CTGF表达显著抑制MDA-MB-231细胞的增殖、侵袭及迁移。Objective To investigate the effects of verteporfin on the proliferation,invasion and migration of human breast cancer MDA-MB-231 cells and the underlying mechanism. Methods MDA-MB-231 cells in the logarithmic growth phase were randomly divided into control group and verteporfin treatment group. After MDA-MB-231 cells were treated with（ 0,4,8,12,16） μmol/m L verteporfin,the minimal inhibitory concentration was determined by CCK-8 assay. After treatment with 4 μmol/m L verteporfin,the invasion and migration abilities of MDA-MB-231 cells were detected by TranswellTMinvasion assay and scratch wound healing assay,respectively. The expression levels of proliferation-associated proteins c-MYC,cyclin D1,Yes-associated protein（ YAP）,cysteine-rich protein 61（ CYR61） and connective tissue growth factor（ CTGF） in MDA-MB-231 cells treated by（ 0,4,8,12,16） μmol/m L verteporfin were determined by Western blotting. Results Verteporfin markedly inhibited the proliferation of MDA-MB-231 cells in a dose-dependent manner,and the minimal inhibitory concentration was 4 μmol/m L. The 4 μmol/m L verteporfin significantly inhibited the invasion and migration abilities of MDA-MB-231 cells.Verteporfin inhibited significantly the expressions of c-MYC,cyclin D1,YAP,CYR61 and CTGF. Conclusion Verteporfin significantly inhibits the proliferation,invasion and migration of MDA-MB-231 cells by down-regulating the expressions of YAP and its target genes CYR61 and CTGF.

关 键 词：维替泊芬 Yes相关蛋白(YAP) MDA-MB-231细胞 增殖 侵袭 迁移 

分 类 号：Q279[生物学—细胞生物学] R965[医药卫生—药理学]