白细胞介素9通过激活信号转导子和转录激活子3(STAT3)通路促进胰腺癌细胞的增殖和迁移  

Interleukin-9 promotes pancreatic cancer cell proliferation and migration via activation of STAT3 pathway

作　　者：黄秋兰[1] 雷荣娥[1] 覃沁怡 覃山羽[1] 姜海行[1] 胡榜利[1] 

机构地区：[1]广西医科大学第一附属医院消化内科,广西南宁530021

出　　处：《细胞与分子免疫学杂志》2017年第9期1228-1233,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(31560257;31360221)

摘　　要：目的探讨白细胞介素9(IL-9)促进胰腺癌细胞的增殖、侵袭及迁移的作用及机制。方法体外培养人胰腺癌PANC-1细胞,实验组予(5、10、20)ng/m L IL-9处理24 h,对照组不予处理。采用实时定量PCR检测PANC-1细胞白细胞介素9受体(IL-9R)的m RNA水平,CCK-8法检测肿瘤细胞的增殖,流式细胞术检测细胞凋亡,TranswellTM实验检测细胞侵袭和迁移能力,Western blot法检测细胞中信号转导子与转录激活子3(STAT3)及磷酸化STAT3(p-STAT3)蛋白水平。STAT3抑制剂AG490预处理前后,进行IL-9处理,再采用以上方法检测细胞增殖情况以及细胞中STAT3和p-STAT3蛋白水平。结果 IL-9处理后,PANC-1细胞IL-9R m RNA水平增高、细胞增殖、侵袭和迁移能力增强,且这种促进作用随着IL-9剂量的升高而增强,但细胞凋亡率无明显改变。与对照组相比,IL-9处理后PANC-1细胞中的p-STAT3蛋白水平显著增加,但STAT3蛋白水平无明显改变。使用AG490预处理后,IL-9对PANC-1细胞增殖的促进作用减弱,且p-STAT3表达水平显著降低。结论 IL-9促进胰腺癌细胞的增殖、侵袭和迁移与STAT3通路激活有关。Objective To investigate the impact of interleukin-9（ IL-9） on proliferation,invasion and migration of pancreatic cancer cells and its mechanism. Methods PANC-1 cel s were cultured in vitro and treated with IL-9 at different concentrations（ 5,10,20 ng/m L） for 24 hours. The level of IL-9 R m RNA was analyzed by quantitative real-time PCR. CCK-8 assay was used to test the proliferation of the cells and flow cytometry to detect the cell apoptosis. TranswellTMassay was employed to determine the invasion and migration of PANC-1 cells. Western blotting was used to detect the STAT3 and p-STAT3 protein expression levels. After PANC-1 cells were treated with different concentrations of STAT3 pathway inhibitor AG490,followed by IL-9 treatment,the STAT3 and p-STAT3 protein expressions,as well as the proliferation of the cells were detected again.Results The level of IL-9 R m RNA and the proliferation rate of PANC-1 cells were enhanced with the increase of IL-9 concentration,and the capacities of cell invasion and migration were promoted significantly. The relative protein expression of p-STAT3 increased greatly in PANC-1 cells after the treatment of IL-9,but STAT3 were not changed significantly compared with the ones without IL-9 treatment. The proliferation-promoting effect of IL-9 on AG490-pretreated PANC-1 cells was induced,and the p-STAT3 protein expression level was notably inhibited. Conclusion The activation of STAT3 pathway is strongly associated with the process that IL-9 mediates the promotion of proliferation, invasion and migration in pancreatic cancer cells.

关 键 词：胰腺癌 PANC-1细胞 白细胞介素9(IL-9) 信号转导子与转录激活子3(STAT3) 

分 类 号：Q279[生物学—细胞生物学] R392-33[医药卫生—免疫学]