寻常型银屑病皮损组织中存活蛋白(survivin)和STAT2的表达增强且二者呈正相关  

The positive relationship between survivin and STAT2 up-expressed in skin lesions of psoriasis vulgaris

作　　者：王昊[1] 冉立伟[2] 惠珂[3] 王新阳[3] 郑焱[1] 

机构地区：[1]西安交通大学第二附属医院皮肤科,陕西西安710004 [2]首都医科大学附属北京朝阳医院西区皮肤科,北京100043 [3]西安交通大学第一附属医院泌尿外科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2017年第9期1261-1265,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30960349)

摘　　要：目的探究凋亡抑制蛋白存活蛋白(survivin)与信号转导子和转录激活子2(STAT2)在寻常型银屑病(PV)患者进行期斑块状皮损组织中的表达及其相关性。方法临床上收集22例PV患者进行期斑块状皮损、非皮损组织以及18例正常皮肤组织。采用实时定量PCR检测皮肤组织中survivin和STAT2 m RNA水平,Western blot法检测皮肤组织中survivin和STAT2蛋白水平;采用免疫组织化学染色法检测皮肤组织中survivin和STAT2表达情况。采用Pearson相关分析确定survivin与STAT2m RNA表达的相关性。结果银屑病皮损组织中survivin、STAT2的m RNA和蛋白水平均高于银屑病非皮损组织和正常皮肤组织,且银屑病皮损组织中survivin、STAT2的m RNA表达呈正相关(r=0.5282)。结论 Survivin、STAT2在寻常型银屑病皮损组织表达增强且二者m RNA表达水平呈正相关。Objective To investigate the expressions of the inhibitor of apoptosis protein survivin and signal transducer and activator of transcription 2（ STAT2） in the tissues of the plaque-like lesions of patients with psoriasis vulgaris（ PV） in the progressive stage and discuss their correlation. Methods We collected the plaque-like lesion samples and non-lesion samples from 22 PV patients and the normal skin sample from 18 healthy controls. Real-time quantitative PCR was used to evaluate the m RNA levels of survivin and STAT2 in these samples. Western blot analysis was performed to determine the protein expressions of survivin and STAT2. Immunohistochemical staining was also used to detect the expressions of survivin and STAT2. The correlation between survivin and STAT2 in the PV lesions was determined by Pearson＇s correlation analysis. Results Compared with healthy control skin and PV non-lesion tissues,the m RNA and protein expressions of survivin and STAT2 were significantly elevated in PV lesion tissues,and the m RNA expression of survivin was positively correlated with STAT2 m RNA in PV lesion tissues（ r = 0. 5282）. Conclusion The expressions of survivin and STAT2 are up-regulated in skin lesions of PV patients,and their m RNA expressions are positively correlated.

关 键 词：寻常型银屑病 存活蛋白(survivin) 信号转导子和转录激活子2(STAT2) 

分 类 号：R758.63[医药卫生—皮肤病学与性病学] R392-33[医药卫生—临床医学]