水稻脆茎突变体cef2的鉴定及基因定位  

Characterizations and Gene Mapping of a Brittle Stalk Mutant cef2 in Rice

作　　者：陆荷微 刘斌美[1] 叶亚峰[1] 陶亮之[1] 吴振宇 范爽[1] 吴跃进[1] 王钰[2] 

机构地区：[1]中国科学院合肥物质科学研究院技术生物与农业工程研究所,合肥230031 [2]安徽大学资源与环境工程学院,合肥230601

出　　处：《分子植物育种》2017年第10期3870-3876,共7页Molecular Plant Breeding

基　　金：中国科学院STS项目(KFJ-EW-STS-083);国家转基因重大科技专项(2014ZX0800935B);国家自然科学青年基金(31301297)共同资助

摘　　要：水稻脆茎突变体是一种重要的种质资源,物理诱变是创建脆茎突变体的一个有效途径。利用重离子辐照扬粳113(yj113)获得一个脆茎突变体cef2,该突变体脆茎特征在抽穗后开始表现,随着成熟度增加脆性特征更为明显。农艺性状分析表明突变体的分蘖数和结实率比野生型显著增加,具有更好的产量潜力。茎秆和叶片的成分测定显示,与野生型相比,cef2突变体茎秆和叶片细胞壁的纤维素含量分别降低24.2%和37.8%,半纤维素含量分别升高18.9%和37.6%,茎秆可溶性糖的含量在抽穗后逐渐降低,成熟期降低了33.6%。cef2茎秆抗折力比野生型减小,减小的程度随着成熟度变化而增加。遗传分析表明,cef2突变体的脆茎特征受单隐性基因控制。利用分子生物学技术将cef2基因定位于水稻第3染色体上SSR标记RM3586和RM487之间,连锁距离分别为5.6 c M和1.4 c M。这些结果为进一步解析cef2脆茎突变性状的分子机理奠定基础。Physical mutati on is an effective way to generate brittle stalk mutants in rice, which are important germplasm resources. In this article, we focused on the potental mechanism of a brittle stalk mutant with temporal specific expression of cef2, generated by heavy ion irradiation of Yang Jing 113. This mutant showed no brittleness difference during vegetative growth. However, significant brittleness was observed after heading. By comparing the cef2 mutant with wild type, we observed differences in agronomic traits, stalk cell wall component, flexural strength and soluble sugar content. The cef2 mutant showed 24.2%, 37.8% less cellulose content and 18.9%, 37.6%higher semi-cellulose content in stalk and leaf in comparing to wild type plant, respectively. On the other hand, the soluble sugar content of cef2 was decreased gradually after heading, the same as stalk breaking-resistant force.Genetic analysis showed that the mutated phenotype of cef2 was controlled by a recessive gene. The cef2 gene was mapped on rice chromosome 3 by using map-based cloning method, and the genetic distances from the gene to the linked SSR markers RM3586 and RM487 were 5.6 c M and 1.4 c M, respectively. The above results laid the basic foundation for the research of the cef2 mutant and its gene cloning.

关 键 词：水稻 脆茎突变体 cef2 细胞壁 基因定位 

分 类 号：Q943.2[生物学—植物学] S511[农业科学—作物学]