盐胁迫条件下耐盐番茄叶片差异蛋白表达分析  

Differential Expression Analysis of Leaf Proteins from Salt-tolerant Tomato under Salt Stress

作　　者：马启林 邓博一 邓用川[1] 钟克焱 夏幽泉[1] 

机构地区：[1]海南大学农林学院,海口570228 [2]东京中央日本语学院

出　　处：《分子植物育种》2017年第10期3893-3899,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31260354;31660381)资助

摘　　要：以导入红海榄总DNA的耐盐番茄及其野生型番茄为材料,于3~4片真叶期分别用调和海水(约1%盐度)和自来水进行灌溉,处理14 d后,采用BPP法提取番茄叶片的全蛋白质,通过等电聚焦和第二向SDSPAGE电泳得到清晰的番茄叶片全蛋白质双向电泳图谱。利用Image Master 2D platinum 6.0软件对海水处理及对照条件下耐盐番茄及野生型番茄差异表达的蛋白进行分析发现,蛋白质双向电泳图谱大约有800个蛋白点,共检测到123个差异蛋白点,每张图谱的匹配率达90%。海水处理下的耐盐番茄与野生型番茄的叶片全蛋白质图谱中共检测到82个高丰度的差异蛋白点,其中42个蛋白点的表达量为上调,40个蛋白点的表达量为下调。对差异表达的蛋白质点进行了基质辅助激光解吸电离串联飞行时间质谱(MALDI-TOF-TOF/MS)鉴定,得到了相关的差异蛋白质信息,为揭示耐盐番茄的耐盐机理、为克隆相关耐盐基因提供了帮助。In this research, we used the salt-tolerant tomato introducing total DNA of Rhizophora stylosa griff. and its wild type tomato as material, irrigated respectively with blended sea water（mixed with sea water and fresh water according to 1：2, about 1.1% salinity） and fresh water for 14 days during three to four true leaf stage, extracted total leaf protein of tomato with BPP method, and got the proteomic profiles of leaves of salt-tolerant tomato and wild types under different treatments by IEF and subsequent SDS-PAGE. The differentially expressed proteins were analyzed using Image Master 2 D platinum 6. 0 software, the result show the proteomic profiles of twodimensional electrophoresis has about 800 protein spots, and find out 123 differential proteins, the matching rate is about 90% among profiles. 82 high abundance differential protein spots, including 42 up-regulated protein spots,40 down-regulated protein spots, were detected in leaf protein electrophoresis atlas between salt-tolerant tomato and its wild type under the condition of sea water irrigation. Through the analysis of the differential protein spots further by MALDI-TOF-TOF/MS, we acquired the relevant information of the differential proteins, which laid the foundation for revealing the mechanism of salt resistance of salt-tolerant tomato and for cloning the gene related to salt tolerance.

关 键 词：番茄 耐盐性 双向电泳 差异蛋白 

分 类 号：S641.2[农业科学—蔬菜学]