甜橙中类AtCIPK23基因的克隆与表达分析  

Cloning and Expression Analysis of AtCIPK23-Like Genes in Citrus sinensis

作　　者：孔佑涵[1] 苑平[1] 张文[1] 李先信[1,2] 吴娟娟 

机构地区：[1]湖南省园艺研究所,长沙410125 [2]湖南大学研究生院隆平分院,长沙410205

出　　处：《分子植物育种》2017年第10期3900-3906,共7页Molecular Plant Breeding

基　　金：湖南省农业科学院科技创新项目(2017JC34);湖南省农业科学院果树品种改良与优质高效栽培技术创新团队(2014td04);湖南省自然科学基金(14JJ6049);湖南省科技重点研发计划(2016NK2195);农业财政资金项目"湘科一号脐橙推广应用"共同资助

摘　　要：钾是柑橘生长发育过程中必需的元素。At CIPK23在拟南芥的钾吸收利用过程中发挥了重要作用,在水稻、杨树和葡萄等多种植物中也发现了与At CIPK23功能相似的CIPK基因,说明类At CIPK23基因在植物进化过程中具有保守性。本研究从甜橙基因组序列信息中分析得到17个柑橘CIPK基因,将其中与At CIPK23基因进化关系较近的Cs2g08190、Cs2g28990和Orange1.1t00555作为柑橘类At CIPK23候选基因;候选基因编码产物与拟南芥CIPK家族成员的氨基酸序列相似性分析结果显示,Cs2g08190与At CIPK23同源性最高(76.8%);三个候选基因均能在细胞质定位,但仅发现Cs2g08190的表达受低钾胁迫诱导表达,推测Cs2g08190是柑橘类At CIPK23基因。Potassium is an essential element in growth and development of Citrus. AtCIPK23 plays an important role in the process of potassium uptake and utilization of Arabidopsis thaliana. CIPK genes whose functions are similar to AtCIPK23 have also been found in many plants, such as rice, poplar and grape, which suggest that AtCIPK23-like genes are conserved in the process of plant evolution. This study obtained 17 Citrus CIPK genes from sweet orange genome sequence analysis, and we selected Cs2g08190, Cs2g28990 and Orange1.1 t00555 whose evolutionary relationships were close to AtCIPK23 genes as the candidate genes. The analysis results of candidate gene coding product and similarity of amino acid sequence of CIPK family members in Arabidopsis thaliana showed that Cs2 g08190 and AtCIPK23 had the highest homology（76.8%）. Three candidate genes could be localized in cytoplasm, however, only the expression of Cs2g08190 was found to be induced by low potassium stress, which suggested that Cs2g08190 was AtCIPK23-like gene in Citrus.

关 键 词：甜橙 钾 CIPK23 柑橘 

分 类 号：Q943.2[生物学—植物学] S666.4[农业科学—果树学]