富贵菜离体快繁及再生体系的建立  

The Establishment of Rapid Propagation in Vitro and Regeneration System of Gynura divaricata DC.

作　　者：李怡 阿拉坦其其格 孙淑英 谢玉杰 贾舒涵 赵永秀[1] 

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021

出　　处：《分子植物育种》2017年第10期3984-3988,共5页Molecular Plant Breeding

基　　金：内蒙古大学2014年度校级创新项目(201414223);内蒙古大学本科毕业论文设计经费共同资助

摘　　要：本研究以富贵菜种苗为实验材料,以富贵菜叶片为外植体,利用植物组织培养技术建立了富贵菜离体快繁体系,对富贵菜再生作了初步研究。结果表明:用4%Na Cl O处理外植体3 min可取得最佳消毒效果,降低外植体的污染率,提高植株成活率和生根率;富贵菜无菌苗在MS培养基上有利于茎叶的生长,在1/2MS培养基上有利于根部的生长;NAA诱导富贵菜生根的最适浓度应在0.05 mg/L左右;富贵菜无菌苗经移栽后,成活率可达到90%左右。富贵菜的叶片接种在MS基本培养基+2.0 mg/L 6-BA+0.5 mg/L NAA中获得愈伤组织后,再接种在MS基本培养基+2.0 mg/L 6-BA+0.2 mg/L NAA+0.4 mg/L KT中获得再生芽。Gynura divaricata DC.＇s seedlings as the experimental material, and leaves were used as explants to obtained rapid propagation system of Gynura divaricata DC. in vitro through tissue culture technology, and some preliminary results have been acquired about the regeneration system of Gynura divaricata DC.. The results showed that 4% Na Cl O treatment of explants could achieve the best disinfection effect, reduce the pollution rate of explants and increase the survival rate and rooting rate of the explants. The sterile seedlings of Gynura divaricata DC. in MS medium were advantageous to the stem and leaf growth. The best concentration of NAA induced rooting was about 0.05 mg/L; the survival rate of the sterile seedlings of Gynura divaricata DC. was about 90%after transplanting, and the survival rate was about 90%. The leaves of Gynura divaricata DC. were inoculated in MS basic medium ＋2.0 mg/L 6-BA＋0.5 mg/L NAA to obtain callus, and then inoculated in MS basic medium＋2.0 mg/L 6-BA ＋0.2 mg/L NAA＋0.4 mg/L KT to obtain regeneration bud.

关 键 词：富贵菜 离体快繁 再生体系 

分 类 号：S567.239[农业科学—中草药栽培]