陈皮半夏通过PI3K-Akt通路对衰老脐静脉内皮细胞中p53和p21表达的影响  

Effects of Chenpi and Banxia on the expression of p53 and p21 by the pathway of PI3K-Akt in aging human umbilical vein endothelial cells

作　　者：黄小波[1] 王宁群[1] 陈玉静[1] 梁伟[1] 赵荣博[1] 陈文强[1] 

机构地区：[1]首都医科大学宣武医院中医科,北京100053

出　　处：《北京中医药》2017年第9期796-799,共4页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81574936);北京市自然科学基金资助项目(7162079)

摘　　要：目的探讨陈皮半夏是否能通过衰老脐静脉内皮细胞(HUVEC)中磷脂酰肌醇3激酶-蛋白激酶B(PI3K-Akt)信号通路对p53和p21的表达进行干预。方法半夏、陈皮按照1∶1的比例配伍,常法煎煮制备陈皮半夏汤剂。对5只SD大鼠使用陈皮半夏汤剂灌胃,分离血清。使用0.25%胰岛素-柠檬酸盐诱导HUVEC衰老,使用流式细胞仪检测细胞周期,建立细胞衰老模型后,通过Western blot法检测陈皮半夏干预衰老HUVEC中PI3K、磷酸化Akt(p-Akt)、p53和p21的表达。结果 0.25%胰岛素-柠檬酸盐诱导可以有效建立细胞衰老模型。与空白对照组和陈皮半夏对照组比较,模型组中PI3K、p-Akt、p53和p21表达显著升高(P<0.01);陈皮半夏和PI3K特异性阻滞剂LY294002干预后,PI3K、p-Akt、p53和p21表达较模型组均显著降低(P<0.05)。结论陈皮半夏有可能通过衰老HUVEC中PI3K-Akt通路调控p53和p21的表达。Object To discuss whether Chenpi and Banxia could intervene the expression of p53 and p21 by the pathway of Phosphatidylinositol-3 kinase（PI3K）-protein kinase B（Akt） in aging human umbilical vein endothelial cells（HUVEC）.Methods Banxia and Chenpi were mixed with a proportion of 1：1,and decocted to make Chenpi Banixia Decoction.The decoction was given to 5 SD rats with gavage administration,and then the serum was isolated.HUVEC senescence were induced by 0.25% insulin citrate,and flow cytometry was used to analyze the cells cycles.After the model of cell senescence was made,the influence of Chenpi and Banxia on the expression of PI3K,p-Akt,p53 and p21 in HUVEC by the way of western blot were detected.Results 0.25% insulin-citrate could be used to induce the senescence of HUVEC.Compared with the control group and Chenpi Banxia control group,the expressions of PI3K,p-Akt,p53 and p21 were significantly increased in the model group（P〈0.01）.Compared with the model group,the expressions of PI3K,p-Akt,p53 and p21 were significantly decreased in Chenpi and Banxia group and LY294002 group（P〈0.05）.Conclusion Chenpi and Banxia could possibly adjust the expressions of p53 and p21 by the pathway of PI3K-Akt in aging HUVEC.

关 键 词：陈皮 半夏 衰老 磷脂酰肌醇3激酶 蛋白激酶B P53 p21 

分 类 号：R285.5[医药卫生—中药学]