通脉方中异黄酮类成分清除DPPH自由基的活性研究  

Study on Scavenging DPPH Free-radical Activity of Isoflavones in Tongmai Formula

作　　者：王付荣 杨雁芳[1] 杨秀伟[1] 

机构地区：[1]北京大学天然药物及仿生药物国家重点实验室,药学院天然药物学系,北京100191

出　　处：《中国现代中药》2017年第11期1545-1549,1554,共6页Modern Chinese Medicine

基　　金：国家科技重大新药创制专项(2009ZX09502-006);“十二五”国家科技支撑计划(2011BAI07B08)

摘　　要：目的:通脉方是由葛根、丹参和川芎3味药按质量1∶1∶1组成的复方。本文研究通脉方中3'-羟基葛根素、3'-甲氧基葛根素、3'-甲氧基大豆苷、3'-羟基泰国野葛根素等25种异黄酮类化合物清除DPPH(2,2'-diphenyl-1-picrylhydrazyl)自由基的活性。方法:通过DPPH自由基清除实验,对25个异黄酮类化合物的体外抗氧化活性进行测定。结果:体外抗氧化研究表明,通脉方中的3'-羟基葛根素、3'-羟基泰国野葛根素、3'-甲氧基葛根素和3'-甲氧基大豆苷具有清除DPPH自由基的活性,呈浓度依赖性,它们的IC50值分别为(9.9±0.6)、(13.7±1.4)、(57.3±1.2)和(66.4±2.3)μmol·L-1。在同样条件下,阳性对照药丁羟甲苯和抗坏血酸的IC50值分别为(75.4±6.9)和(16.5±1.6)μmol·L-1。结论:本研究结果能够预测3'-羟基葛根素、3'-羟基泰国野葛根素、3'-甲氧基葛根素和3'-甲氧基大豆苷可能是通脉方生物活性物质基础的重要组成部分。Objective： Tongrnai formula（ TMF） is a drug combination of three components including Puerariae Loba- tae Radix [ roots of Pueraria lobata（ Willd. ） Ohwi ], Salviae Mihiorrhizae Radix（ roots of Salvia miltiorrhiza Bge. ） and Chua- nxiong Rhizoma（ rhizomes of Ligusticum chuanxiong Hort. ） in a weight ratio of 1 ： 1 ： 1. The scavenging activity against DPPH free-radical by isoflavonoid compounds isolated from TMF were studied in this paper. The assayed isoflavonoid compounds in- clude daidzein, formononetin, 5-hydroxylononin, ononin, daidzin, 3＇-methoxypuerarin, genistin, puerarin, formononetin- 8-C-β-D-apiofuranosyl-（1---＆ ） -O-fl-D-glueopyranoside, formononetin-7-O-β-D-apiofuranosyl-（1→6 ） -O-fl-D-glueopyranoside, laneeolarin, kakkanin, daidzein-7, 4＇-di-O-β-D-glucopyranoside, mirificin, 3＇-hydroxypuerarin, 3＇-methoxydaidzin, form- ononetin-8-C-fl-D-xylopyranosyl-（ 1→ 6 ） -O-β-D-glucopyranoside , genistein-8-C-β-D-apiofuranosyl-（ 1 → 6 ） -O-β-D-glucopyr- anoside, genistein-7-O-β-D-apiofuranosyl-（ 1 -→6 ） -O-β-D-glueopyranoside （ ambocin ）, 3＇-hydroxymirificin, 6＂-O-β-D-xylo- sylpuerarin, bioehanin-A-8-C-β-D-apiofuranosyl-（1→6 ）-O-β-D-glucopyranoside, 3＇-methoxydaidzein-7, 4＇-di-O-fl-D-gluco- pyranoside, daJdzein-7-O-fl-D-glucopyranosyl-（ 1 → 4 ）-O-β-D-glueopyranoside, and daidzein-7-O-α-D-glueopyranosyl-（ 1 4）-O-β-D-glueopyranoside. Methods： The in vitro antioxidant potentials of above-mentioned 25 isoflavonoid compounds were evaluated by DPPH（2, 2＇-diphenyl-l-picrylhydrasyl）free-radical scavenging test. Results： In vitro antioxidant studies showed that 3＇-hydroxypuerarin, 3＇-hydroxymirificin, 3＇-methoxypuerarin, and 3＇-methoxydaidzin in TMF possessed notable antioxi- dant effects on DPPH free-radical scavenging in a concentration-dependent manner and their values of IC50 were （9. 9 ± 0. 6 ）, （ 13.7 ± 1.4）, （57. 3 ± 1.2）, and（66.4 ±2. 3） μmol -L-1 , respectively. While the I

关 键 词：通脉方 异黄酮 3'-羟基葛根素 3'-羟基泰国野葛根素 3'-甲氧基葛根素 3'-甲氧基大豆苷 DP-PH自由基 

分 类 号：R282.71[医药卫生—中药学]