文冠果壳抗氧化活性及抑制人HepG2细胞增殖活性部位筛选  

Antioxidant Activity of Fruit Shells of Xanthoceras sorbifolia and Screening of Active Components Inhibiting Proliferation of HepG_2 Cell Lines

作　　者：张严磊 肖鸿飞 施欢贤 宋忠兴 唐志书 

机构地区：[1]陕西中医药大学/陕西省中药资源产业化协同创新中心,陕西省中药基础与新药研究重点实验室,陕西咸阳712083

出　　处：《中国现代中药》2017年第11期1572-1574,1581,共4页Modern Chinese Medicine

基　　金：陕西省协同创新计划项目(2015xt-52);陕西省科技统筹创新工程计划项目(2016KTTSSF01-06-01)

摘　　要：目的:筛选文冠果壳的抗氧化及抑制肝癌HepG2细胞增殖活性部位。方法:用水,10%、30%、50%、70%、95%乙醇水溶液从文冠果中提取得到A、B、C、D、E和F提取物,用清除自由基DPPH·能力筛选抗氧化活性部位,用MTT法筛选抑制肝癌细胞增殖的活性部位。结果:6个提取部位均有抗氧化活性和抑制Hep G2细胞增殖作用,其中E(70%乙醇水提取)部位的抗氧化活性最强,当质量浓度为0.2 mg·m L-1时,其对DPPH·清除率能达到70.82%;提取部位F(95%乙醇水提取)抑制Hep G2细胞增殖作用最强,当其质量浓度为75μg·m L-1时,抑制率高达70.1%。结论:文冠果壳提取物具有抗氧化及抑制人Hep G2增殖活性,具有开发前景。Objective： To screen the activity part of the antioxidant and inhibiting the proliferation of HepG2 cell line from the fruit shells of Xanthoceras sorbifolia. Methods： The fruit shells of X. sorbifolia were extracted by water, 10% , 30%, 50%, 70% and 95% ethanol aqueous, respectively, and obtained six different parts named as A, B, C, D, E and F. These fractions were assayed by scavenging the DPPH ~ free-radical to determine their antioxidant activity and inhibiting the proliferation against HepG2 cell line by MTF method. Results： All the extracts of the fruit shells of X. sorbifolia showed the an- tioxidant activity and inhibiting the proliferation of HepG2 cells. Among them, part D （ the extract of 70% ethanol aqueous） showed the strongest activity for scavenging the DPPH free-radical, and when its concentration was 0. 2 mg · mL- 1, the capaci- ty of antioxidant activity could go up to 70. 82%. While the part E possessed the best anti-proliferation activity against HepG2 cells, and its inhibition reached 70. 10%. Conclusion： In this paper we studied the anti-proliferation activity against HepG2 cells and antioxidant activity of different solvent extract from the fruit shells of X. sorbifolia, and provided the theory basis for potential use of the fruit shells of X. sorbifolia.

关 键 词：文冠果壳 抗氧化 抑制肝癌细胞 筛选 

分 类 号：R273.57[医药卫生—中西医结合]