PACl投加对SBBF效能及菌群结构的影响  

Influence of PACl addition on performance and microbial communities in SBBF

作　　者：季斌[1] 秦慧 陈威[1] 杨开[2] 

机构地区：[1]武汉科技大学城市建设学院,湖北武汉430065 [2]武汉大学土木建筑工程学院,湖北武汉430072

出　　处：《华中科技大学学报（自然科学版）》2017年第11期127-132,共6页Journal of Huazhong University of Science and Technology(Natural Science Edition)

基　　金：湖北省教育厅中青年人才资助项目(Q20161116);武汉科技大学青年人才资助项目(2016xz033)

摘　　要：研究了聚合氯化铝(PACl)对序批式生物膜滤池(SBBF)处理生活污水的强化作用,包括去除效能的影响和基于454焦磷酸测序的微生物多样性分析.研究结果表明:PACl投加可使得SBBF获得满意的碳氮磷去除效果,同时高通量测序结果有助于认识铝盐对废水生物处理系统微生物的影响;50mg·L^(-1)的PACl投加可以使出水的总磷(TP)去除量从48.7%显著提高至68.2%,TP浓度低于0.5mg·L^(-1),对氮和化学需氧量(COD)去除的影响较小;454焦磷酸测序分析揭示铝胁迫下SBBF的微生物的多样性和丰度;铝盐投加可以使得浮游菌属(Planctomyces)、消化链球菌属(Peptostreptococcaceae)和嗜冷菌属(Psychrobacter)的丰度减少,假单胞菌属(Pseudomonas)和梭状芽孢杆菌属(Clostridium)的丰度增加,而不动杆菌属包括tjernbergiae不动杆菌(Acinetobacter tjernbergiae)和鲁菲不动杆菌(Acinetobacter lwoffii)一直处于主导地位.Poly-aluminum chloride（PACl）addition to a sequencing batch biofilm filter（SBBF）was investigated for domestic sewage treatment.The removal performance was evaluated and the microbial diversity was studied by 454pyrosequencing.Research results show that the SBBF could achieve satisfactory phosphorus removal with PACl addition and the pyrosequencing results contribute to understand the impacts of toxic aluminum on microbial communities in wastewater treatment systems.Addition of 50mg·L（-1 ）PACl could enhance phosphorus removal from 48.7%to 68.2%with the total phosphorus（TP）in effluent below 0.5 mg·L（-1）,while the nitrogen and chemical oxygen demand（COD）removal is slightly affected.454pyrosequencing analyses reveal the microbial diversity and richness abundance of bacterial clusters in the SBBF under aluminum stress.Bacterial abundance of Planctomyces,Peptostreptococcaceae and Psychrobacter decreases,while genus Pseudomonas and Clostridiumincreases,and the dominant position of the genus Acinetobacter,including Acinetobacter tjernbergiae and Acinetobacter lwoffii stays the same.

关 键 词：铝毒 菌群结构 协同除磷 序批式生物膜滤池 高通量测序 

分 类 号：X703[环境科学与工程—环境工程]