碧江乌头内生真菌Alternaria solani次生代谢产物研究  

Study on the Secondary Metabolites of Endophytic Fungus Alternaria Solani from Aconitum Tsaii

作　　者：李桂琼[1] 郭成鑫 黄丽[1] 段晓燕[1] 刘王艳 何丹[1] 沈勇[1] 

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201

出　　处：《中国药师》2017年第10期1760-1764,共5页China Pharmacist

基　　金：国家自然科学基金项目(编号:21362045)

摘　　要：目的:对碧江乌头内生真菌Alternaria solani次生代谢产物进行研究。方法:将内生真菌Alternaria solani发酵产物用甲醇提取,回收甲醇至浸膏状,浸膏用乙酸乙酯萃取,得粗提物。粗提物用各种柱色谱进行分离纯化,经光谱数据(~1H-NMR,^(13)C-NMR,MS)进行结构鉴定。结果:分离得到12个化合物,分别鉴定为:(22E,24R)-Ergosta-4,6,8(14),22-tetraen-3-one(1),1,3-二油酸甘油酯(2),5-(3',3'-Dimethylallyloxy)-3-methoxy-4-methylphthalide(3),拟盘多毛孢H2-倍半萜内酯(4),过氧麦角甾醇(5),7-dehydroxyl-zinniol(6),麦角甾-7,22-二烯-3β,5,6-三醇(7),9-octadecenoic acid-2',3'-dihydroxy propyl ester(8),8-甲基塔拉胺(9),查斯曼宁(10),滇乌碱(11),crassicauline A(12)。结论:从内生真菌Alternaria solani中分离得到12个化合物,其中化合物9~12均为首次分离得到。Objective：To study the secondary metabolites of endophytic fungus Alternaria solani from Aconitum Tsaii. Methods：The endogenous fungui fermentation product was extracted by methanol followed by recycling methanol to obtain the extract, and the ex-tract was extracted by ethyl acetate to obtain the crude extract. The compounds were isolated and purified by column chromatography and identified based on the spectral analysis （ MS, 1 H-NMR and 13 C-NMR） . Results：Totally 12 compounds were isolated from A. so-lani and characterized as （22E, 24R）-Ergosta-4, 6, 8 （14）, 22-tetraen-3-one （1）, 1,3-Diolein （2）, 5-（3＇, 3＇-Dimethylallyloxy）-3-methoxy-4-methylphthalide （3）, pseudomonas aeruginosa H2-sesquiterpene lactone （4）, ergosterol peroxide （5）, 7-dehydroxyl-zinni-ol （6）, ergosterol-7, 22-diene-3β, 5,6-triol （7）, 9-octadecenoic acid -2＇,3＇-dihydroxy propyl ester （8）, 8-O-Methyltalatisamine （9）, Chasmanine （10）, yunaconitine （11） and crassicauline A （12）. Conclusion：Totally 12 compounds are isolated from the endo-phytic fungus Alternaria solani, and four of them are isolated for the first time.

关 键 词：碧江乌头 内生真菌 次生代谢产物 

分 类 号：R284.1[医药卫生—中药学]