精料中直链与支链淀粉比对羔羊瘤胃细菌菌群的影响  

Effects of Amylose to Amylopectin Ratio in Concentrate on Rumen Bacterial Community in Lambs

作　　者：于洋洋[1] 赵芳芳[1,2] 张爱忠 姜宁[1] 李雁冰[1] 陈勇[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]扬州大学动物科学与技术学院,扬州225009

出　　处：《动物营养学报》2017年第11期3951-3961,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金面上项目(31372338)

摘　　要：本文旨在研究精料中直链与支链淀粉比对不同日龄羔羊瘤胃细菌菌群的影响。选取48只体重无显著差异(P>0.05)的初生公羔,随机分成4组(每组3个重复,每个重复4只),各组精料中直链与支链淀粉比分别为0.12(木薯淀粉组)、0.23(玉米淀粉组)、0.24(小麦淀粉组)和0.48(豌豆淀粉组)。试验全期共77 d。于羔羊21、35、56和77日龄时,每个重复随机选择1只羔羊屠宰,采集瘤胃液样品。通过PCR-变性梯度凝胶电泳(DGGE)技术测定分析羔羊瘤胃细菌菌群的多样性。结果表明,不同直链与支链淀粉比精料组的羔羊于21、35、56和77日龄时,瘤胃细菌多样性指数、均匀度和丰富度均无显著性差异(P>0.05)。21日龄羔羊瘤胃细菌多样性指数显著高于35日龄(P<0.05),56和77日龄羔羊瘤胃细菌多样性指数有所升高,但在数值上仍低于21日龄羔羊。DGGE回收的条带多属厚壁菌门及拟杆菌门。56日龄的小麦淀粉组羔羊瘤胃内牛单胞月形菌含量较高。由此可见,不同直链与支链淀粉比精料未影响羔羊瘤胃优势细菌的结构,但饲喂小麦淀粉饲粮可促进56日龄羔羊瘤胃内牛单胞月形菌的生长。The objective of this study w as to determine the effects of amylose to amylopectin ratio in concentrate on rumen bacterial community in lambs at different days of age. Forty-eight male lambs w ith no significant difference in body w eight（ P〈0. 05） w ere randomly divided into 4 groups,there w ere 3 replicates per group w ith 4 lambs per replicate. The concentrate amylose to amylopectin ratio of different groups w as 0.12（ tapioca starch）,0.23（ corn starch）,0.24（ w heat starch） and 0.48（ pea starch）,respectively. The w hole experiment period w as 77 days. One lamb from each replicate w ere randomly selected and slaughtered at 21,35,56 and77 days of age to collect rumen fluid sample. The diversity of rumen bacterial community in lambs w as determined by PCR-denaturing gradient gel electrophoresis（ DGGE）. The results show ed that there w as no effect on diversity index,evenness and richness of lambs at 21,35,56 and 77 days of age（ P〈0.05）. Diversity index in lambs at 21 days of age w as significant higher than that at 35 days of age（ P〈0.05）,and it w as increased at 56 and 77 days of age,but the numerical values w ere still low er than that at 21 days of age. Bands recovered from DGGE w ere almost belonged to Bacteroidetes and Firmicutes. Selenomonas bovis content in rumen in lambs at56 days of age in w heat starch group w as higher. Therefore,different amylose to amylopectin ratio in concentrate do not affect the structure of lamb rumen dominant bacteria,but feed w heat starch diet can promote the grow th of Selenomonas bovis in rumen in lambs at 56 days of age.

关 键 词：直链与支链淀粉比 羔羊 PCR-变性梯度凝胶电泳 瘤胃细菌菌群 多样性指数 

分 类 号：S826[农业科学—畜牧学]