环境pH对褐环粘盖牛肝菌转录组的影响  

Effect of environmental pH on Suillus luteus transcriptome

作　　者：杨威 蒋莹 陈有君[1] 闫伟[1] 李立民[1] 霍峰 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010020

出　　处：《广东农业科学》2017年第8期47-54,173,共9页Guangdong Agricultural Sciences

摘　　要：为了探明环境酸碱条件对褐环粘盖牛肝菌基因表达的影响,测序分析了生长在不同p H环境下的该菌转录组。结果发现,与生长在p H3环境下的菌丝相比,p H4、4.5、5、5.5、6、7、8条件下的菌丝,分别有743、870、1 177、1 512、1 628、3 135、2 902个基因发生了差异表达,这些差异表达基因在基因本体论数据库中分别注释到2 029、2 368、2 733、3 125、3 168、4 626、4 728个功能,在京都基因与基因组百科全书中分别定位到76、81、108、121、116、180、177个代谢途径,其中内质网对蛋白质的加工、泛醌和其他类萜醌的生物合成途径、谷胱甘肽代谢途径、ABC transporter途径、色氨酸代谢途径和抗生素生物合成途径等途径的富集程度较高。说明环境酸碱度对褐环粘盖牛肝菌蛋白质加工及物质跨膜转运等具有很大影响。In order to explore the influence of environmental acid-base conditions on gene expression of Suillus luteus,we sequenced the transcriptome of the strain grown in different pH environment.The result showed that,compared with the hyphae grown at pH 3,the hyphae at pH 4,4.5,5,5,5,6,7 and 8 had 743,870,1 177,1 512,1 628,3 135 and 2 902 genes differentially expressed respectively.These differential expressed genes were respectively annotated to 2 029,2 368,2 733,3 125,3 168,4 626 and 4 728 functions in gene ontology,and set to 76,81,108,121,116,180 and 177 metabolic pathways in the Kyoto Genome and Genome Encyclopedia.Among them,the degree of enrichment is higher in protein processing on endoplasmic reticulum,ubiquinone and other like terpene quinone biosynthetic pathway,glutathione metabolism pathway,ABC transporter pathway,tryptophan metabolic pathway,antibiotic biosynthetic pathway and other ways,indicating that environmental acid-base conditions have significant effects on protein processing and transmembrane transport of S.luteus.

关 键 词：基因表达 代谢途径 褐环粘盖牛肝菌 酸碱条件 

分 类 号：S646.3[农业科学—蔬菜学]