福寿螺组织细胞原代培养及染色体核型分析  

Primary histocyte culture and chromosome karyotype analysis of Pomacea canaliculata

作　　者：李绪杰 张福周 邹湘辉[1] 杨东娟[1] 查广才[1] 

机构地区：[1]韩山师范学院食品工程与与生物科技学院,广东潮州521041

出　　处：《广东农业科学》2017年第8期127-132,共6页Guangdong Agricultural Sciences

基　　金：中国科学院环境化学与生态毒理学国家重点实验室开放基金(KF2016-28);广东省大学生科技创新培育专项(pdjh2017b0330);广东省教育厅科技创新项目(2013KJCX 0126);潮州市科技引导计划项目(2014SF02)

摘　　要：无菌分离取得福寿螺肌肉、外套膜、肾脏及脑组织,分别采用胰酶消化法和研磨法进行组织细胞分离,选取DMEM、F12和改良1640培养基分别进行细胞原代培养,光镜下观察细胞的生长状态,MTT法测定细胞活性。细胞培养一定时间后进行染色体制备及染色体核型分析。结果表明,福寿螺组织细胞在不同的培养基培养下活力具有明显差异,用含水解乳蛋白的DMEM培养基培养的福寿螺外套膜和斧足组织细胞活力较好;用M199培养基培养的福寿螺斧外套膜和脑组织细胞活力较好。核型分析结果表明福寿螺染色体为二倍体(2n=28,NF=56),核型为2B型,核型不对称系数为66.4%。Pomacea canaliculata's muscle,mantle,kidney and brain tissues were aseptic separated by trypsin digestion and grinding method.Cells were primary cultured with DMEM,F12 and modified 1640 medium.The growing status was observed under light microscope.The cell viability was determined by MTT method.After a certain time,chromosome preparation and chromosome karyotype analysis were conducted.Results showed that there was significant difference in histocytes viability among different culture medium.The better viability of mantle and muscle histocytes was cultured with DMEM medium containing hydrolyzed milk protein.The better viability of mantle and brain histocytes was cultured with M199 medium.In karyotype analysis,results showed that P.canaliculata was diploid(2n = 28,NF = 56).The nuclear type was 2B and the nuclear asymmetry coefficient was 66.4%

关 键 词：福寿螺 原代培养 染色体 核型分析 

分 类 号：S917.4[农业科学—水产科学]