山西地区汉族人群两种稀有血型系统基因多态性研究  

Study the gene polymorphism of two rare blood groups in Han nationalities in Shanxi area

作　　者：李敏[1] 周永安[2] 李超[1] 谢林峰 杨书艳[2] 刘培贤[2] 贾彩虹[2] 田树雄 侯霞[1] 

机构地区：[1]山西医科大学研究生院,太原030001 [2]山西医科大学第二医院,太原030001

出　　处：《中国优生与遗传杂志》2017年第11期28-30,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究山西地区汉族人群红细胞MNS、Kell两种稀有血型系统基因多态性分布特点,为人类群体遗传学、临床用血和自身输血及山西地区稀有血型库的建立奠定基础。方法本研究采用序列特异性引物-聚合酶链式反应(SSP-PCR)法,对150名山西地区汉族人群两种稀有血型系统进行基因分型,并进行统计学分析。结果山西地区汉族人群MNS、Kell两种稀有血型基因频率依次M=0.5533,N=0.4467,S=0.0367,s=0.9633,K1=0.0167,K2=0.9833,χ2检验比较基因型分布的观察值与期望值发现,检测结果符合Hardy-Weinberg平衡法则。结论山西地区汉族人群MNS稀有血型系统抗原基因频率分布呈多态性,而Kell稀有血型系统抗原基因频率分布呈单态性,且基因频率基本符合Hardy-Weinberg遗传平衡法则。Objective：To study the gene polymorphism of MNS、Kell blood group in Han nationalities in Shanxi area and build the basis for human population genetics,clinical use of blood or blood auto-transfusion and rare blood bank. Methods：Two blood groups genetic typing of 150 Shanxi adults were detected by sequence specific primers-polymerase chain reaction（PCR-SSP）method and analyzed the data by SPSS 19.0. Results：The allele gene frequencies of two rare blood groups in Han nationalities of Shanxi area were M=0.5533,N=0.4467,S=0.0367,s=0.9633,K1=0.0167,K2=0.9833. Results from chi-square test showed that the observed value and expected value of genetic typing were in line with the law of Hardy-Weinberg equilibrium（P〉0.05）. Conclusion：The distribution of MNS gene frequency in Han nationality in Shanxi area showed a polymorphism state and those of Kell showed a single state. The gene frequency was in line with the law of Hardy-Weinberg equilibrium.

关 键 词：稀有血型 PCR-SSP 基因分型 基因频率 

分 类 号：R457.11[医药卫生—治疗学]