JSI-124特异性阻断STAT3通路对宫颈癌SiHa细胞增殖、凋亡相关研究  

Specific Blockage of STAT3 Signaling Pathway with JSI-124 Suppresses Growth of Cervical Carcinoma Line SiHa

作　　者：马丽君[1] 赵桂治[1] 杨伟[1] 梁佩枫 黑常春[2] 

机构地区：[1]宁夏回族自治区人民医院妇产科,宁夏银川750004 [2]宁夏医科大学,宁夏银川750004

出　　处：《药品评价》2017年第22期31-31,34,35,36,37,共5页Drug Evaluation

基　　金：宁夏自然科学基金;项目计划编号:NZ15195

摘　　要：目的:观察JSI-124特异性阻断STAT3通路对宫颈癌Si Ha细胞增殖抑制以及胞质转录因子STAT3蛋白表达的影响。方法:MTT法检测不同浓度JSI-124不同干预时间对Si Ha细胞增殖的影响;流式细胞术检测不同浓度JSI-124对Si Ha细胞干预24小时凋亡的情况;Western blot法检测细胞中STAT3蛋白及其磷酸化蛋白表达的变化。采用SPSS11.5统计学分析软件对数据进行单因素方差分析及t检验。结果:JSI-124可以抑制宫颈癌Si Ha细胞的增殖,干预效果随药物作用时间及药物浓度增加而增强;JSI-124可诱导宫颈癌Si Ha细胞的凋亡,并具有较强的浓度依赖关系;经JSI-124干预后的宫颈癌Si Ha细胞,STAT3蛋白的表达无明显变化,而磷酸化激活状态的STAT3蛋白表达显著降低(P<0.01)。结论:选择性JAK/STAT3抑制剂JSI-124可明显抑制宫颈癌Si Ha细胞的增殖并诱导其细胞凋亡。其可能成为宫颈癌防治的一条新的有效途径。Objective: To investigate the effect of specific blockage of STAT3 signaling pathway with JAK/STAT3 selective inhibitor JSI-124 suppresses growth of Cervical Carcinoma Carcinoma Line Si Ha and protein expression of cytoplasmic transcription factor STAT3. Methods: Si Ha was treated with JSI-124 in different concentration, and then cell proliferation was examined by MTT, cell apoptosis was detected by flow cytometry. The protein expressions of Cervical Carcinoma Carcinoma Line Si Ha were detected by Western blot analysis with anti-STAT3 and other antibody. The data were analyzed by one-way ANOVA test and t test using SPSS11.5 software package. Results: The cell proliferation was apparent decreased with the treatment of JSI-124, and which is concentrations and time dependent. The cell apoptosis was obviously increased by different concentration JSI-124 for 24 hours. The protein expression of anti-STAT3 has no change, but anti-phospho-specific STAT3 was significantly decreased. Conclusion: The JAK/STAT3 selective inhibitor JSI-124 can inhibit cell proliferation and encourage cell apoptosis in Si Ha. Therefore, it might be a powerful treatment option for cervical carcinoma.

关 键 词：JSI-124 STAT3 SIHA细胞 细胞增殖 细胞凋亡 

分 类 号：R96[医药卫生—药理学]