不同标本测定儿童25-羟基维生素D的比对分析及偏移评估  

Comparison on the results of 25-hydroxyvitamin D determinations with different specimen types in children and their biases

作　　者：周后钢[1] 毛安华[1] 谭浩[1] 

机构地区：[1]湖南省妇幼保健院检验科,湖南长沙410008

出　　处：《检验医学》2017年第11期1036-1038,共3页Laboratory Medicine

摘　　要：目的分析儿童末梢血与静脉血血浆25-羟基维生素D[25(OH)D]水平的差异及可比性,为2种标本25(OH)D检测结果的互认提供依据。方法采用酶联免疫吸附试验分别检测85例儿童末梢血和静脉血血浆25(OH)D水平,采用直线回归分析计算2种标本检测结果的相关系数、直线回归方程以及在医学决定水平处的偏移。结果末梢血25(OH)D水平明显低于静脉血(P<0.01)。相关性分析显示,静脉血25(OH)D/末梢血25(OH)D比值与静脉血血细胞比容(HCT)末梢血HCT比值呈明显正相关(r=0.634,P<0.01)。末梢血和静脉血25(OH)D水平显著相关(r=0.995),各医学决定水平处的偏移均在试剂盒给定的误差范围内。结论末梢血血浆25(OH)D水平低于静脉血,但结果具有较好的可比性,临床上可以采集末梢血检测25(OH)D。Object ive To analyze the di f ferences and comparabil i ty of 25-hydroxyvi tamin D [25 （OH） D] determination results in peripheral blood and venous blood,and to provide a reference for the homogeneity of 25（OH）D results determined by the 2 specimen types. MethodsThe levels of 25 （OH） D of peripheral blood and venous blood were determined by enzyme-linked immunosorbent assay. Linear regression analysis was used to analyze the correlation and bias of 25（OH）D determination results in the 2 specimen types. Results The levels of 25（OH）D of peripheral blood were lower than those of venous blood（P〈0.01）. The ratio of peripheral blood 25（OH）D/ venous blood 25（OH）D was positively correlated with the ratio of peripheral blood hematocrit（HCT）/ venous blood HCT（r=0.634,P〈0.01）. There was a correlation for the levels of 25（OH）D between peripheral blood and venous blood（r=0.995）. The bias of 25 （OH）D levels between the 2 specimen types was in allowed error provided by kits. Conclusions The levels of 25（OH）D of peripheral blood are lower than those of venous blood,and their results are comparable. Peripheral blood can be used to determine 25（OH）D levels.

关 键 词：25-羟基维生素D 偏移 末梢血 静脉血 

分 类 号：R446.1[医药卫生—诊断学]