死亡谷芽胞杆菌NBIF-001防治番茄棒孢叶斑病研究  

Control effect of Bacillus vallismortis NBIF-001 on Corynesporaleaf spot of tomato

作　　者：黄大野 贲海燕[2] 曹春霞 王开梅 饶犇 刘晓艳 万中义 杨自文 

机构地区：[1]湖北省生物农药工程研究中心,武汉430064 [2]黑龙江省农业科学院园艺分院,哈尔滨150069

出　　处：《植物保护》2017年第6期192-195,共4页Plant Protection

基　　金：湖北省自然科学基金重点项目(2016ABA103);国家重点研发计划(SQ2017ZY060083);湖北省农业科技创新中心创新团队项目(2016-620-000-001-038)

摘　　要：为明确死亡谷芽胞杆菌NBIF-001菌株对棒孢叶斑病的防治效果,采用平板对峙结合显微观察确定其对多主棒孢的抑菌活性,并采用活体盆栽试验研究该菌株对番茄棒孢叶斑病防治效果。结果表明,在平板对峙条件下NBIF-001对多主棒孢具有良好的抑菌活性,显微镜下观察到菌丝发生扭曲、肿胀和变形;盆栽试验结果表明NBIF-001对棒孢叶斑病的防治效果达到71.46%。NBIF-001和50%啶酰菌胺WG联用防治番茄棒孢叶斑病的效果明显好于单独施用50%啶酰菌胺WG,具有较明显的增效作用,化学杀菌剂50%啶酰菌胺WG对NBIF-001菌株生长无明显影响,两者具有较好的相容性。In order to evaluate control effect of Bacillus vallismortis NBIF-001 on Corynesporaleaf spot,the inhibition activity was tested by dual culture method and microscope observation,and the control efficacy was investigated by pot experiment.The results showed that the strain NBIF-001 had high inhibition activity in dual culture,hypha deformed and cytoplasmic vacuoles enlarged.Pot experiment demonstrated that NBIF-001 exhibited a better control effect on Corynespora leaf spot of tomato with the efficacy of 71.46%.NBIF001 combined with boscalid 50% WG had obvious synergic effect on the disease control.Bocalid showed no significant effect on the growth of NBIF-001,thus they both had the good compatibility.

关 键 词：死亡谷芽胞杆菌 棒孢叶斑病菌 生防效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.412[农业科学—植物保护]