棉花品种SSR标记变性与非变性PAGE检测法的比较研究  

Comparison of Denatured and Non-Denatured PAGE Detection Method on Cotton SSR Markers

作　　者：刘国栋[1] 王芙蓉[1] 张传云[1] 陈煜[1] 张景霞[1] 杜召海 张军[1] 

机构地区：[1]山东棉花研究中心,山东济南250100

出　　处：《山东农业科学》2017年第11期131-133,共3页Shandong Agricultural Sciences

基　　金：山东省自然科学基金青年基金项目(ZR2014CQ047);国家棉花产业技术体系项目(CARS-18-10);泰山学者建设工程专项(ts201511070)

摘　　要：本研究以12份棉花品种为材料,用三对多态性丰富的SSR引物对变性和非变性PAGE检测法进行比较分析。结果表明,两种检测方法鉴定单株个体基因型的类型一致;变性PAGE检测法检测结果清晰,但操作过程繁琐;非变性PAGE检测法操作相对简单、检测通量高,更适用于材料的高通量检测分析。Three SSR primer pairs with high polymorphism and 12 cotton varieties were used to comparatively analyze the denatured and non-denatured PAGE detection methods. The results indicated that the type of individual genotype which was identified by the two detection methods was consistent. The testing results of denatured PAGE were more clear,but its operation process was complex. The operation process of non-denatured PAGE was simple and it had high test flux,which was more suitable for high throughput detection analysis.

关 键 词：棉花 SSR 聚丙烯酰胺凝胶电泳 银染 

分 类 号：S562[农业科学—作物学]