宫颈分泌物与盆腔液解脲支原体与沙眼衣原体检测在输卵管性不孕患者感染评估中的应用  

Application of detection of Ureaplasma urealyticum and Chlamydia trachomatis in cervical secretion and pelvic fluid in the evaluation of infection in tubal infertility patients

作　　者：武卫华[1] 郝翠芳[2] 田丽娟[1] 王雪楠[1] 牛焕付[1] 

机构地区：[1]山东济宁医学院附属医院生殖医学科,山东济宁272000 [2]山东烟台毓璜顶医院生殖医学科,山东烟台264000

出　　处：《中华医院感染学杂志》2017年第22期5216-5219,共4页Chinese Journal of Nosocomiology

基　　金：山东省自然科学基金资助项目(ZR2010HM040)

摘　　要：目的研究探讨宫颈分泌物与盆腔液解脲支原体(Uu)、沙眼衣原体(Ct)检测在输卵管性不孕患者感染评估中的应用价值。方法选取2015年7月-2016年9月于医院检测的输卵管性不孕患者72例,设置为试验组;另抽取同期来院体检的健康人员31名作为参考组,实施聚合酶链反应(PCR)法检测宫颈分泌物与盆腔液内Uu、Ct,分析患者感染率。结果试验组患者宫颈分泌物中Uu、Ct检出率分别为30.56%、5.56%,显著高于对照组的9.68%、0.00%,试验组患者盆腔液中Uu检出率为4.17%,显著高于对照组的0.00%(P<0.05);继发性输卵管性不孕患者宫颈分泌物中Uu、Ct检出率显著高于原发性输卵管性不孕者,继发性输卵管性不孕患者盆腔液中Uu检出率显著高于原发性输卵管性不孕者(P<0.05);经药敏检测,Uu对强力霉素的敏感率为100.00%、交沙霉素的敏感率为96.00%,对四环素、红霉素敏感率最低。结论输卵管性不孕患者与Uu、Ct感染有着密切关联性,建议加强Uu、Ct检测,对Uu、Ct感染者可采取药敏试验,选择合理的抗菌药物治疗,抑制Uu、Ct对输卵管的持续性损害。OBJECTIVE To investigate the effects of detection of Ureaplasma urealyticum（Uu）and Chlamydia trachomatis（Ct）in cervical secretion and pelvic fluid in the evaluation of infection in tubal infertility patients. METHODS A total of 72 cases of fallopian tubal infertility patients from Jul.2015 to Sep.2016 were selected and set as observation group,and another 31 health persons were selected as control group.The polymerase chain re-action（PCR）method was used for detection of Uu and Ct in cervical secretion and pelvic fluid,and the infection rates were analyzed.RESULTS The detection rates of cervical secretion Uu and Ct in observation group of patients were 30.56% and 5.56%,which were significantly higher than 9.68% and 0.00% in control group.The detection rate of pelvic fluid Uu in observation group of patients was 4.17%,which was significantly higher than 0.00% in control group（P〈0.05）.The detection rates of Uu and Ct in cervical secretion of patients with secondary tubal infertility were higher than those of primary tubal infertility,and the detection rate of pelvic fluid Uu in patients with secondary tubal infertility was higher than that of primary tubal infertility（P〈0.05）.The drug sensitivity test showed that,the sensitive rate of Uu to doxycycline was 100%,to josamycin was 96%,and to tetracycline and erythromycin were the lowest.CONCLUSION Patients with tubal infertility are closely related to Uu and Ct infection,so it is proposed to strengthen the Uu and Ct detection,take the drug sensitivity test in patients with Uu and Ct infection,select reasonable antibacterial drugs and prevent the Uu and Ct from continuous damage of fallopian tubes.

关 键 词：宫颈分泌物 盆腔液 输卵管性不孕 解脲支原体 沙眼衣原体 感染 

分 类 号：R375[医药卫生—病原生物学]