常春藤药材HPLC指纹图谱及8种成分的含量测定方法  

HPLC fingerprint and quantitative analysis of 8 components from Hedera helix

作　　者：刘家鑫[1] 陈明明[2] 杨雪艳 张欣 赵云丽[1] 于淼[1] 于治国[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳军区药品仪器检验所,辽宁沈阳110026

出　　处：《沈阳药科大学学报》2017年第11期979-986,1032,共9页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省自然科学基金资助项目(2015010756-301);2015年辽宁省大学生创新创业训练项目

摘　　要：目的以不同产地的常春藤为研究对象,采用高效液相色谱法建立常春藤药材的指纹图谱及其8种指标性成分的含量测定方法,为科学评价和控制常春藤药材的质量提供科学依据。方法采用高效液相色谱法,指纹图谱的建立与含量测定的建立:色谱柱为Extend-C18柱(150 mm×4.6 mm,5 m),以体积分数为0.05%的磷酸溶液和乙腈进行梯度洗脱,检测波长为210 nm。结果建立了常春藤药材的指纹图谱和8种成分的含量测定方法,确定了常春藤药材指纹图谱中的21个共有峰,不同产地的15批药材中各成分的含量为0.01%~4.4%。结论本方法为科学评价常春藤药材质量提供了依据。Objective To establish the fingerprint of Hedera helix from different areas and quantitation method of eight kinds of indicator components by high performance liquid chromatography（HPLC）,whichprovided scientific basis for scientific evaluation and control of the quality of Hedera helix. Method The analysis was performed on Poroshell 120 SB-C18（150 mm × 4. 6 mm,5 μm） for fingerprint and quantitative analysis,and 0. 05% phosphoric acid solution and acetonitrileare as mobile phase in gradient mode. The detection wavelength was set at 210 nm. Results A HPLC method was developed for fingerprint and quantitation of Hedera helix,and 21 common peaks in Hedera helix were confirmed. The contents of 8 compounds in Hedera helix in 15 batches of different habitats are ranged from 0. 01%-4. 4%. Conclusion The method provides the advantage of repeatability and specificity to evaluate the quality of Hedera helix.

关 键 词：常春藤 指纹图谱 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]