全程与间断推拿对延缓失神经大鼠骨骼肌萎缩的实验研究  

Experimental study on the postponed effects of whole-and intermittent-way massage on skeletal muscle atrophy in denervated rats

作　　者：陆永嘉[1] 严隽陶[2] 马书杰[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属岳阳中西医结合医院推拿科,上海200437

出　　处：《上海中医药杂志》2017年第10期97-99,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81373764);国家自然科学基金青年基金资助项目(81603713)

摘　　要：目的探讨全程与间断推拿手法联合跑台训练对延缓失神经支配骨骼肌萎缩的效应。方法 40只SD大鼠随机分为正常对照组、失神经对照组、全程推拿组和间断推拿组,每组10只。除正常对照组外,其余3组建立坐骨神经失神经模型,造模后休整3周。全程推拿组给予2个月捏法手法及跑台训练,每天1次;间断推拿组先给予1个月捏法手法及跑台训练,每天1次,后期静养1个月。各组于干预后2个月检测腓肠肌湿重比和细胞因子(IGF-I/b FGF/Myo D)阳性面积。结果与正常对照组比较,失神经对照组各细胞因子明显升高(P<0.05);腓肠肌湿重比明显降低(P<0.05)。与间断推拿组和失神经对照组比较,全程推拿组细胞因子明显降低(P<0.05);腓肠肌湿重比明显升高(P<0.05)。结论全程推拿手法联合跑台训练可以一定程度上延缓失神经支配骨骼肌萎缩。Objective To discuss the postponed effects of whole- and intermittent-way massage manipulation combined with treadmill training on denervated skeletal muscle atrophy. Methods 40 SD rats were randomly divided into the normal control group, denervation group, whole-way massage group and intermittent-way massage group, 10 rats in each group. Except the normal control group, the denervation model of sciatic nerve was established in the other groups. After modeling, no treatments were performed for 3 weeks. The whole-way massage group was treated with pinching manipulation and treadmill training once a day for 2 months, and the intermittent-way massage group was treated with pinching manipulation and treadmill training once a day for one month and then rested for one month. After intervention, the gastrecnemius wet weight ratio was detected, and the positive areas of cytokines including insulin-like growth factor-I （ IGF-I ）, basic fibroblast growth factor（bFGF）and myogenic differentiation factor（MyoD） were determined. Results Compared with the whole-way massage group, the levels of cytokines were obviously increased in the intermittent-way massage group （ P 〈 0.05 ）, and the gastrecnemius wet weight ratio was obviously decreased （ P 〈 0.05 ）. Conclusion The whole-way massage manipulation combined with treadmill training can delay the denervated skeletal muscle atrophy in a certain extent.

关 键 词：推拿 跑台 骨骼肌萎缩 细胞因子 大鼠 

分 类 号：R244.1[医药卫生—针灸推拿学]