白癜风患者血清免疫球蛋白、补体、自身抗体检测及分析  

Detection and Analysis of Immunoglobulins,Complements and Autoantibody Levels in the Sera of Vitiligo Patients

作　　者：刘成刚[1] 杨慧 李建标[1] 任曲[1] 朱美财[1] 

机构地区：[1]空军总医院临床检验中心,北京100142

出　　处：《标记免疫分析与临床》2017年第11期1201-1204,共4页Labeled Immunoassays and Clinical Medicine

基　　金：基金项目:首都临床特色应用研究(编号:Z11117058811086)

摘　　要：目的检测白癜风患者血清中免疫球蛋白IgG、IgA、IgM,补体C3、C4,抗核抗体(ANA)、抗甲状腺过氧化物酶抗体(A-TPO)、抗甲状腺球蛋白抗体(A-TG)的水平并进行比较分析。方法收集门诊确诊为白癜风的患者血清168例和正常体检人群血清88例,性别、年龄分布无统计学差异,采用速率散射比浊法检测白免疫球蛋白IgG、IgA、IgM和补体C3、C4,采用ELISA法检测抗核抗体,采用电化学发光免疫分析法检测A-TPO、A-TG,结果用SPSS19.0统计软件进行分析。结果白癜风患者组血清中免疫球蛋白IgG、IgA、IgM,补体C3、C4表达水平与正常人对照组差异不具有统计学意义(P>0.05);白癜风组与正常对照组ANA阳性率分别为8.1%和5.7%,经比较差异不具有统计学意义(P>0.05);两组间A-TPO的阳性率分别为18.8%和10.2%,差异有统计学意义(P<0.05);两组A-TG阳性率分别为25.8%和4.5%,经比较差异具有统计学意义(P<0.01)。结论部分白癜风患者存在体液免疫紊乱,在发病机制上可能与自身免疫性甲状腺疾病有相似之处;免疫球蛋白、补体及抗核抗体等免疫指标的检测在白癜风诊治中评价的意义不大。Objective Detection and analysis of the immunoglobulins IgG, IgA, IgM, complementsC3, C4, antinuclear antibody （ANA）, antibody against thyroid peroxidase （A-TPO） and antibody against thyroglobulin （A-TG） in the sera of vitiligo patients. Methods 168 cases of serum samples of vitiligo patients and 88 cases of normal samples from physical examination were collected, there were no statistical difference in the gender and the age distribution between the two groups. Rate scattering turbidimetric method was used to detect the immunoglobulins IgG,IgA, IgM, complements C3, C4 levels ; ELISA method was used to detect the ANA levels, while A-TPO, A-TG levels were detected by using Electrochemilum inescence analysis method, and the results were analyzed by SPSS19.0 statistical software. Results No statistical difference were found in levels of immunoglobulins IgG, IgA, IgM, complements C3, C4 between the vitiligo patients and the normal control group. The ANA positive rate in vitiligo patients and the normal control group were 8.1% and 5.7 % respectively, and no statistical difference by comparison （ P 〉 0.05 ）. The A- TPO positive rate in patients with vitiligo were higher than that of normal controls （P 〈 0.05 ）, they were 18.8 % and 10.2% respectively. The A- TG positive rate in two groups are 25.8 % and 4.5 % respectively, the difference was strongly different by comparison （P 〈 O. 001 ）. Conclusion Part of the vitiligo patients may be suffering from the humoral immune disorder, and their pathogenesis may be related to autoimmune thyroid diseases; The values of immunoglobulin ,Complements and ANA have no apparent application values in the treatment and evaluation of vitiligo patients.

关 键 词：白癜风 免疫球蛋白 补体C3 C4 抗核抗体 抗甲状腺过氧化物酶抗体 抗甲状腺球蛋白抗体 

分 类 号：R446.6[医药卫生—诊断学] R758.41[医药卫生—临床医学]