CD4CD8细胞及凝血调节因子与复发性自然流产的临床研究  

Clinical Study of CD4/CD8 Cells and Coagulation Factor in Recurrent Spontaneous Abortion

作　　者：韩利蓉 宋江勤 郭飞波 李光梅 张栋武 

机构地区：[1]湖北省天门市第一人民医院检验科,湖北天门431700 [2]广东吴川市妇幼保健院,广东吴川524500 [3]广东医科大学附属高明医院,广东佛山528500

出　　处：《标记免疫分析与临床》2017年第11期1225-1228,共4页Labeled Immunoassays and Clinical Medicine

基　　金：湛江市医学类非资助科技攻关项目(编号:2016B101)

摘　　要：目的研究不明原因反复流产患者蜕膜组织CD4、CD8免疫细胞的局部表达及与TM、PAI-1、F_(1+2)、D-二聚体等凝血相关因子的关联性,为不明原因流产患者治疗提供帮助。方法采用细胞免疫芯片技术对不明原因流产患者50例子宫蜕膜组织进行CD4、CD8细胞计数并计算CD4/CD8比值;酶联免疫吸附试验方法测定患者血浆血栓调节蛋白(TM)、纤溶酶原激活抑制物-1(PAI-1)、凝血酶原片段F1+2;全自动免疫荧光分析法测定D二聚体(D-dimer,D-D)因子浓度。同时以正常妊娠无流产征兆主动要求人工流产的50人为对照组,比较上述指标在两个群体中的表达,并进行对比分析研究。结果与对照组比较,患者蜕膜组织CD4水平及CD4/CD8比值均下降,CD8水平呈高表达,血浆TM、PAI-1、F1+2因子呈高表达,D-D呈低表达状态。两组比较差异均有统计学意义(P<0.05)。结论反复流产患者蜕膜组织局部免疫功能表达异常,血液处于一定程度的高凝状态而纤溶系统受损。修复上述状态可对治疗不明原因反复流产患者提供新思路。Objective To study the local expression of membrane tissue CD4, CD8 immune cells and TM, PAI- 1, F1 ＋ 2, D- dimers coagulation related factors in unexplained recurrent spontaneous abortion patients and their relevance, so to provide help for the treatment of patients with unexplained abortion. Methods The CD4/ CD8 cells were counted and the CD4/CD8 ratio was calculated by immunocytochemistry. Using enzyme- linked immunosorbent assay and adsorption test method in 50 patients with unexplained abortion （ experimental group, non- pregnant） for plasma thrombomodulin （ TM ）, plasminogen activator inhibitor- 1 （PAI-1）, prothrombin fragment F1＋2, D dimer （D-dimer, D-D） and the normal pregnancy factor concentration, and 50 women （ control group） as control, the expression of the above two groups factors in two groups were measured, and then compared the results. Results Compared with the control group, TM, PAI-1 and F1＋2 were highly expressed,and the expression of D-D was low. The difference between the two groups was statistically significant （ P 〈 0.05 ）. Conclusion The blood of patients with recurrent abortion was in a state of high coagulation and the fibrinolytic system was damaged. Restoration of the above status provides new ideas for the treatment of unknown causes.

关 键 词：CIM CD8 反复流产 TM PAI-1 F1+2 D-D 

分 类 号：R446.6[医药卫生—诊断学] R714.21[医药卫生—临床医学]