炎症分子核因子κB及骨桥蛋白在骨关节炎患者膝关节液中的表达及临床意义  

Expression of NF-κB and Osteopontin in the Knee Joint Fluid of Patients with Osteoarthritis and Its Clinical Significance

作　　者：蒋华[1] 曾勇[1] 马红兵[1] 徐兵[1] 王俊瑞[1] 

机构地区：[1]成都市第二人民医院骨科,四川成都610000

出　　处：《标记免疫分析与临床》2017年第11期1293-1297,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的分析炎症分子核因子κB及骨桥蛋白在骨关节炎患者膝关节液中的表达及临床意义。方法选取46例采取人工全膝关节置换术治疗的骨关节炎患者作为观察组,另选同期的46例外伤截肢、半月板损伤、交叉韧带断裂、盘状软骨损伤等行手术治疗的患者作为对照组,分别采用免疫组化法、酶联免疫吸附试验(ELISA)检测观察组与对照组患者膝关节液中的炎症分子核因子κB及骨桥蛋白的表达水平,根据骨关节炎诊治指南(2007年版)对骨关节炎患者分为早期、中期、晚期,比较观察组与对照组、不同临床分期骨关节炎患者膝关节液中炎症分子核因子κB及骨桥蛋白的表达水平,采用Spearman相关分析炎症分子核因子κB及骨桥蛋白与骨关节炎临床分期、炎症分子核因子κB与骨桥蛋白的相关性。结果观察组膝关节液中的炎症分子核因子κB及骨桥蛋白表达水平均高于对照组,差异具有统计学意义(P<0.05);不同临床分期骨关节炎患者膝关节液中炎症分子核因子κB及骨桥蛋白的表达水平差异有统计学意义(P<0.05),炎症分子核因子κB及骨桥蛋白的表达水平随着临床分期增加而提高;经Spearman相关分析,炎症分子核因子κB及骨桥蛋白与骨关节炎临床分期均呈正相关(r=0.714、0.498,P<0.05),炎症分子核因子κB与骨桥蛋白呈正相关(r=0.512,P<0.05)。结论骨关节炎患者膝关节液中的炎症分子核因子κB及骨桥蛋白表达水平显著提高,骨桥蛋白可能通过炎症分子核因子κB信号转导途径参与骨关节炎的发生、发展,根据两者的表达水平来判断病情的严重程度具有积极作用。Objective To analyze the expression of inflammatory cytokine kappa B （κB ） and osteopontin in knee joint fluid of patients with osteoarthritis and its clinical significance. Methods 46 patients with osteoarthfitis treated with total knee arthroplasty were selected as the observation group. 46 patients with traumatic amputation, meniscus injury, cruciate ligament rupture and discoid cartilage injury were treated as control group osteopontin in the knee joint fluid of the observation group and the control group were detected by immunohistochemistry and enzyme-linked immunosorbent assay （ELISA） respectively. According to the guidelines for the diagnosis and treatment of osteoarthritis （2007 edition）, patients with osteoarthritis were divided into early stage, middle stage and late stage. Spearman correlation analysis was used to analyze inflammation molecular nuclear factor KB （ NF-κB ）, osteopontin, osteoarthritis clinical stage, the correlation between inflammatory molecules NF-κB and osteopontin. Results The levels of NF-κB and osteopontin in the knee joint of patients with osteoarthritis were significantly higher than those in the control group（P 〈0.05 ）. The expression of NF-κB in the knee joint fluid of patients with osteoarthritis was significantly different among different clinical stages （ P 〈 0.05 ）. The expression of NF-κB and osteopontin increased with the increase of clinical stage. The expression of NF-κB and osteopontin was higher than that of osteopontin （ r = 0.714,0. 498, P 〈 0.05 ）. There was a positive correlation between inflammatory cytokine kappa B and osteopontin （ r = 0.512, P 〈 0.05 ）. Conclusion The expression of NF-κB and osteopontin in the knee joint of patients with osteoarthritis significantly increase. The osteopontin might be involved in the occurrence and development of osteoarthritis through the inflammatory cytokine κB signal transduction pathway. The level of expression has a positive effect on the severity of the condition.

关 键 词：骨关节炎 炎症分子核因子κB 骨桥蛋白 膝关节液 

分 类 号：R684.3[医药卫生—骨科学]