miR-137对肾癌GRC-1细胞增殖与凋亡的影响  

Effect of miR-137 on Proliferation and Apoptosis of Renal Carcinoma GRC-1 Cells

作　　者：董艳[1] 肖有文 董建华[1] 方梅容 胡祥[1] 刘颖[1] 鲍永强[1] 潘红霞[1] 李婷[1] 何玲[1] 

机构地区：[1]乐山市人民医院肾内科,四川乐山614000

出　　处：《标记免疫分析与临床》2017年第11期1306-1309,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的研究miR-137对肾癌GRC-1细胞增殖与凋亡的影响。方法采用miR-137模拟物转染至肾癌GRC-1细胞,实验分为未转染组(未进行miR-137模拟物转染)、miR-137对照组(转染随机序列)、miR-137转染组(进行miR-137模拟物转染)。荧光定量PCR检测各组转染后48h细胞中miR-137表达水平,应用噻唑蓝细胞增殖试验(MTT)、流式细胞检测技术与免疫荧光评价miR-137对肾癌GRC-1细胞增殖和凋亡的影响。结果转染miR-137模拟物48h后,荧光定量PCR检测发现未转染组、miR-137对照组及miR-137转染组GRC-1细胞中miR-137的相对表达量依次为(24.43±2.03)%、(26.57±2.55)%、和(73.30±3.29)%,差异有统计学意义(P<0.05)。MTT细胞增殖实验与流式细胞仪检测结果显示,与未转染组、miR-137对照组相比,miR-137转染组转染48h后肾癌GRC-1细胞的增殖率显著降低,凋亡率显著升高,差异有统计学意义(P<0.05),而未转染组、miR-137对照组肾癌GRC-1细胞的增殖率、凋亡率比较差异无统计学意义(P>0.02)。结论 miR-137可明显抑制肾癌GRC-1细胞增殖和促进其凋亡,故有望成为肾癌基因治疗的新靶点。Objective To study the effect of miR- 137 on proliferation and apoptosis of renal carcinoma GRC- 1 cells. Methods miR-137 analogies were transfected into renal carcinoma GRC-1 cells. The non-transfection group（without transfection of miR-137 analogies）, the miR-137 control group （transfected with random sequence） and the miR-137 transfection group （with transfection of miR-137 analogies） were used. The expression of miR-137 in cells was detected by fluorescence quantitative PCR in 48h after transfection. The effect of miR-137 on proliferation and apoptosis of renal carcinoma GRC-1 cells was evaluated with the methyl- thiazolyl- tetrazolium （MTT） test, flow cytometry and immunofluorescence. Results 48 hours after the transfection of miR-137 analogies, fluorescence quantitative PCR detected that the relative expression levels of miR-137 in GRC-1 cells in the non-transfection group, the miR-137 control group and the miR-137 transfection group were （24.43 ＋ 2.03 ） %, （26.57 ＋ 2.55 ） % and （73.30 ＋ 3.29 ） %, respectively （ P 〈 0.05 ）. MTT cell proliferation assay and flow cytometry showed that the proliferation rate of renal carcinoma GRC-1 cells was significantly lower and the apoptosis rate was significantly higher in the miR-137 transfection group than the non-transfection group and miR-137 control group at 48 hours after transfection （P 〈0.05 ）. However,there was no significant difference between the non-transfection group and miR-137 control group in the proliferation rate and apoptosis rate of renal carcinoma GRC- 1 cells （ P 〉 0. 02 ）. Conclusion miR- 137 can inhibit the proliferation and promote the apoptosis of GRC- 1 cells, which might be a new target for gene therapy of renal carcinoma.

关 键 词：miR-137 肾癌 GRC-1细胞 增殖 凋亡 

分 类 号：R737.11[医药卫生—肿瘤]